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Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency

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dc.contributor.authorMcKinley, Endyaen
dc.contributor.authorSpeckhart, Savannah L.en
dc.contributor.authorKeane, Jessica A.en
dc.contributor.authorOliver, Mary A.en
dc.contributor.authorRhoads, Michelle L.en
dc.contributor.authorEdwards, J. Lannetten
dc.contributor.authorBiase, Fernando H.en
dc.contributor.authorEaly, Alan D.en
dc.date.accessioned2024-02-01T14:32:50Zen
dc.date.available2024-02-01T14:32:50Zen
dc.date.issued2023-12-21en
dc.date.updated2024-01-10T14:50:00Zen
dc.description.abstractThis work explored whether supplementing selective members of the interleukin-6 (IL6) cytokine family during in vitro bovine oocyte maturation affects maturation success, cumulus&ndash;oocyte complex (COC) gene expression, fertilization success, and embryo development potential. Human recombinant proteins for IL6, IL11, and leukemia inhibitory factor (LIF) were supplemented to COCs during the maturation period, then fertilization and embryo culture commenced without further cytokine supplementation. The first study determined that none of these cytokines influenced the rate that oocytes achieved arrest at meiosis II. The second study identified that LIF and IL11 supplementation increases <i>AREG</i> transcript abundance. Supplementation with IL6 supplementation did not affect <i>AREG</i> abundance but reduced <i>HAS2</i> transcript abundance. Several other transcriptional markers of oocyte competency were not affected by any of the cytokines. The third study determined that supplementing these cytokines during maturation did not influence fertilization success, but either LIF or IL11 supplementation increased blastocyst development. No effect of IL6 supplementation on subsequent blastocyst development was detected. The fourth experiment explored whether each cytokine treatment affects the post-thaw survivability of cryopreserved IVP blastocysts. None of the cytokines supplemented during oocyte maturation produced any positive effects on post-thaw blastocyst re-expansion and hatching. In conclusion, these outcomes implicate IL11 and LIF as potentially useful supplements for improving bovine oocyte competency.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationMcKinley, E.; Speckhart, S.L.; Keane, J.A.; Oliver, M.A.; Rhoads, M.L.; Edwards, J.L.; Biase, F.H.; Ealy, A.D. Influences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competency. Animals 2023, 14, 44.en
dc.identifier.doihttps://doi.org/10.3390/ani14010044en
dc.identifier.urihttps://hdl.handle.net/10919/117802en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcowen
dc.subjectoocyteen
dc.subjectinterleukinen
dc.subjectcytokineen
dc.subjectin vitro embryo productionen
dc.titleInfluences of Supplementing Selective Members of the Interleukin-6 Cytokine Family on Bovine Oocyte Competencyen
dc.title.serialAnimalsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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Journal Articles, Multidisciplinary Digital Publishing Institute (MDPI)
Scholarly Works, School of Animal Sciences