Polygalacturonate lyase activity in Bacteroides from the human colon
| dc.contributor.author | Chastain, Jane L. | en |
| dc.contributor.department | Food Science and Technology | en |
| dc.date.accessioned | 2021-03-03T18:46:29Z | en |
| dc.date.available | 2021-03-03T18:46:29Z | en |
| dc.date.issued | 1983 | en |
| dc.description.abstract | In the course of studies on degradation of dietary fiber by the human colon bacteria, a number of Bacteroides strains from the human colon have been found to degrade polygalacturonic acid and/or pectin. Four of these strains were examined for presence of polygalacturonate lyase (PGAL). The Bacteroides strains studied synthesized inducible, cell-associated PGALs when the bacteria were grown on media containing polygalacturonic acid or on peanut cell wall (a prototype dietary fiber with a uronide content of 14.0%) as the sole carbon source. No PGAL activity could be detected in cultures grown on media containing glucose or D-galacturonate. The PGALs produced by the four Bacteroides strains had properties similar to other bacterial PGALs: An alkaline pH optima (8.5), stimulation of activity by calcium ions. complete inhibition by ethylenediaminetetraacetic acid (EDTA), preference for polygalacturonic acid as a substrate over pectin, and accumulation of unsaturated products which absorb at 235 nm. The Bacteroides PGALs catalyzed partial degradation of the uronides of peanut cell wall substrate, but at a slower rate compared to their action on polygalacturonic acid substrate. | en |
| dc.description.degree | M.S. | en |
| dc.format.extent | v, 60 leaves | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/10919/102592 | en |
| dc.language.iso | en | en |
| dc.publisher | Virginia Polytechnic Institute and State University | en |
| dc.relation.isformatof | OCLC# 10185572 | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject.lcc | LD5655.V855 1983.C427 | en |
| dc.subject.lcsh | Bacteroides | en |
| dc.title | Polygalacturonate lyase activity in Bacteroides from the human colon | en |
| dc.type | Thesis | en |
| dc.type.dcmitype | Text | en |
| thesis.degree.discipline | Food Science and Technology | en |
| thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
| thesis.degree.level | masters | en |
| thesis.degree.name | M.S. | en |
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