Development and characterization of cell-free amino acid incorporation systems from Cellvibrio gilvus

dc.contributor.authorKo, Thong-Sungen
dc.contributor.committeechairBarnett, Lewis B.en
dc.contributor.committeememberAckerman, C. J.en
dc.contributor.committeememberBunce, George Edwinen
dc.contributor.committeememberCampbell, T. Colinen
dc.contributor.committeememberKing, Kendall W.en
dc.contributor.departmentBiochemistry and Nutritionen
dc.date.accessioned2014-03-14T21:29:58Zen
dc.date.adate2010-02-16en
dc.date.available2014-03-14T21:29:58Zen
dc.date.issued1967-02-15en
dc.date.rdate2010-02-16en
dc.date.sdate2010-02-16en
dc.description.abstractA ribosomal system has been derived from <u>Cellvibrio gilus</u> which is active with respect to amino acid incorporation into hot trichloroacetic acid-insoluble material. Optimal incorporation systems showed a requirement for ribosomes, pH 5 enzyme protein, ATP plus ATP-generating system, magnesium ion, ammonium ion, and GTP. ¹⁴C-phenylalanine incorporation was stimulated by poly U in the presence of t-RNA which has been derived from this bacterium. The t-RNA had some activation effect on poly U-directed ¹⁴C-phenylalanine incorporation, whereas no effect on natural m-RNA primed ¹⁴C-leucine incorporation was observed. A complementary unlabeled amino acid mixture inhibited ¹⁴C-leucine incorporation. ¹⁴C-leucine incorporation was greatly inhibited by RBAase and puromycin, moderately inhibited by chloramphenicol, but not effected significantly by DNAase and actinomycin D. The optimum pH and temperature for ¹⁴C-leucine incorporation were 7.8 and 300° C, respectively. Polyamiaes (putrescine, spermine, and spermidine) had a slight stimulatory effect on poly U-directed.¹⁴C-phenylalanine incorporation, but no effect on ¹⁴C-Leucine incorporation.en
dc.description.degreeMaster of Scienceen
dc.format.extent93, 2 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-02162010-020558en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-02162010-020558/en
dc.identifier.urihttp://hdl.handle.net/10919/41212en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1967.K57.pdfen
dc.relation.isformatofOCLC# 34044396en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectamino acidsen
dc.subject.lccLD5655.V855 1967.K57en
dc.titleDevelopment and characterization of cell-free amino acid incorporation systems from <u>Cellvibrio gilvus</u>en
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Nutritionen
thesis.degree.grantorVirginia Polytechnic Instituteen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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