Studies of a low molecular weight Zn-containing protein population of lens tissue
| dc.contributor.author | Schwab, Susan J. | en |
| dc.contributor.department | Biochemistry and Nutrition | en |
| dc.date.accessioned | 2018-04-24T19:14:27Z | en |
| dc.date.available | 2018-04-24T19:14:27Z | en |
| dc.date.issued | 1982 | en |
| dc.description.abstract | Maintenance of reduced protein sulfhydryl groups is an important function of lens metabolism. In an attempt to inter-relate how lens sulfhydryl metabolism, low molecular weight peptides and trace molecular weight Zn-containing protein population was studied. The concentration of rate lens Zn-containing protein population was studied. The concentration of rat lens Zn decreased 30% from day 10 to day 35 postpartum. After 6 weeks on a low-Zn diet, rats had 25% less lens Zn than control groups. Selenite-induced cataract did not affect lens Zn concentration. Of the total lens Zn, 25% was recovered in the ultrafiltration fraction of less than 20,000 molecular weight which contained 1% of the total soluble protein. Lens tissue has low molecular weight TCA-soluble components that can bind Hg. Isoelectric points of low molecular weight protein fractions were between pI 5.2-5.5. Polypeptide molecular weight was determined to be less than 5,000 daltons by gel filtration chromatography. Aggregation on SDS-polyacrylamide gel electrophoresis yielded apparent higher molecular weights of these proteins. Although the proteins isolated had metallothionein-like character in that they were of low molecular weight, contained Zn and had acidic pI values, the paucity of cysteine residues indicates that metallothionein is not a component of low molecular weight Zn-containing lens proteins. | en |
| dc.description.degree | Master of Science | en |
| dc.format.extent | vi, 82, [1] leaves | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.uri | http://hdl.handle.net/10919/82893 | en |
| dc.language.iso | en_US | en |
| dc.publisher | Virginia Polytechnic Institute and State University | en |
| dc.relation.isformatof | OCLC# 9403512 | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject.lcc | LD5655.V855 1982.S368 | en |
| dc.subject.lcsh | Crystalline lens | en |
| dc.title | Studies of a low molecular weight Zn-containing protein population of lens tissue | en |
| dc.type | Thesis | en |
| dc.type.dcmitype | Text | en |
| thesis.degree.discipline | Biochemistry and Nutrition | en |
| thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
| thesis.degree.level | masters | en |
| thesis.degree.name | Master of Science | en |
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