Neuropathologic Effects of Phenylmethylsulfonyl Fluoride (PMSF)-Induced Promotion and Protection inn Organophosphorus Ester-Induced Delayed Neuropathy(Opidn) in Hens

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dc.contributor.authorMassicotte, Christiane IIen
dc.contributor.committeechairJortner, Bernard S.en
dc.contributor.committeememberEhrich, Marion F.en
dc.contributor.committeememberInzana, Karen D.en
dc.contributor.committeememberJortner, Bernard S.en
dc.contributor.departmentSmall Animal Clinical Sciencesen
dc.date.accessioned2011-08-06T16:06:18Zen
dc.date.adate1998-04-08en
dc.date.available2011-08-06T16:06:18Zen
dc.date.issued2000-05-12en
dc.date.rdate1999-04-08en
dc.date.sdate1998-03-30en
dc.description.abstractThe serine/cysteine protease inhibitor phenylmethylsulfonyl fluoride (PMSF) has been used both to promote and to protect against neuropathic events of organophosphorus-induced delayed neuropathy (OPIDN) in hens (Lotti et al., 1991; Veronesi et al., 1985; Pope and Padilla, 1990; Pope et al., 1993). This study expands upon this work by correlating clinical and neuropathological findings in these modifications of OPIDN. To provide appropriate models of OPIDN, single phenyl saligenin phosphate (PSP) dosages of 0.5, 1.0, or 2.5 mg/kg were administered to adult hens. PMSF (90 mg/kg) was given either 4 hours after or 12 hours prior to PSP administration. Clinical signs and pathologic changes in the biventer cervicis nerve (El-Fawal et al., 1988) were monitored. PSP alone, 2.5 mg/kg, ellicitated severe OPIDN (terminal clinical score 7.5 & ± 1.0 [0-8 scale]; neuropathology score 2.7 ± 0.3 [0-4 scale, based on myelinated fiber degeneration]). PMSF given 12 hours prior to PSP gave complete protection (clinical and neuropathology scores of 0; p<0.0001). Signs and lesions of OPIDN were absent following 0.5 mg/kg PSP alone, but PMSF given 4 hours after PSP potentiated its neurotoxic effects (clinical score 4.0 ± 0.0; neuropathology score 3.5 ± 0.3; p<0.0001). At the time of sacrifice, there was a correlation (r = 0.61) between the clinical score on the last day of observation and the neuropathology scores (p<0.0001). This study demonstrates that the intensity of peripheral nerve myelinated fiber degeneration correlates with clinical deficits in PMSF-induced potentiation and protection in OPIDN.en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.otheretd-3798-154753en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-3798-154753en
dc.identifier.urihttp://hdl.handle.net/10919/10100en
dc.publisherVirginia Techen
dc.relation.haspartCMassicotteIntro.pdfen
dc.relation.haspartCMassicotteMain.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectToxicologyen
dc.subjectOrganophosphateen
dc.subjectNeuropathyen
dc.subjectPromotionen
dc.titleNeuropathologic Effects of Phenylmethylsulfonyl Fluoride (PMSF)-Induced Promotion and Protection inn Organophosphorus Ester-Induced Delayed Neuropathy(Opidn) in Hensen
dc.typeThesisen
thesis.degree.disciplineSmall Animal Clinical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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