Detection of Q129H Immune Escape Mutation in Apparently Healthy Hepatitis B Virus Carriers in Southwestern Nigeria

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dc.contributor.authorAdesina, Olufisayo Adeyemien
dc.contributor.authorAkanbi, Olusola Aanuoluwapoen
dc.contributor.authorOpaleye, Oladele Oluyinkaen
dc.contributor.authorJaphet, Margaret Oluwatoyinen
dc.contributor.authorWang, Boen
dc.contributor.authorOluyege, Adekemi Olubukunolaen
dc.contributor.authorKlink, Patrycjaen
dc.contributor.authorBock, C.-Thomasen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.date.accessioned2021-07-09T18:28:29Zen
dc.date.available2021-07-09T18:28:29Zen
dc.date.issued2021-06-29en
dc.date.updated2021-07-08T14:24:06Zen
dc.description.abstractAs the global effort to eradicate hepatitis B continues, immune escape mutations (IEMs) and drug resistance mutations (DRMs) affecting its diagnosis, treatment, and prevention are compromising this goal. However, knowledge about the prevalence and circulation of these mutations in Nigeria is scarce. Serum samples (<i>n</i> = 199) from apparently healthy prospective blood donors, pregnant women, and individuals presenting with fever in southwestern Nigeria were analyzed for the presence of IEMs and DRMs by means of nested PCR in the HBV S (HBs) and HBV polymerase (Pol) genes, followed by phylogenetic and mutational analyses. In total, 25.1% (<i>n</i> = 50/199) of samples were positive for HBV, as measured by PCR. In 41 samples (20.6%), both fragments could be amplified, whereas the HBs gene and the Pol gene fragment alone were detected in 0.5% (<i>n</i> = 1/199) and 4% (<i>n</i> = 8/199) of samples, respectively. Sequences were successfully obtained for all 42 HBs gene fragments but for only 31/49 Pol gene fragments (totaling 73 sequences from 44 individuals). All sequences were identified as HBV genotype E. IEMs were present in 18.2% (<i>n</i> = 8/44) of the sequences of HBV-positive individuals with available sequences. IEM Q129H was detected in eight out of the 44 (18.2%) HBV isolates sequenced in this study; however, no DRMs were observed. This study confirms the circulation of HBV IEMs and reports the presence of Q129H IEM for the first time in Nigeria. Intensified research on the dynamics of IEM is necessary in order to enhance the elimination of HBV.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAdesina, O.A.; Akanbi, O.A.; Opaleye, O.O.; Japhet, M.O.; Wang, B.; Oluyege, A.O.; Klink, P.; Bock, C.-T. Detection of Q129H Immune Escape Mutation in Apparently Healthy Hepatitis B Virus Carriers in Southwestern Nigeria. Viruses 2021, 13, 1273.en
dc.identifier.doihttps://doi.org/10.3390/v13071273en
dc.identifier.urihttp://hdl.handle.net/10919/104133en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjecthepatitisen
dc.subjectmutationen
dc.subjectresistanceen
dc.subjectPCRen
dc.subjectphylogenyen
dc.subjectNigeriaen
dc.titleDetection of Q129H Immune Escape Mutation in Apparently Healthy Hepatitis B Virus Carriers in Southwestern Nigeriaen
dc.title.serialVirusesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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