Glyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion of Lactobacillus reuteri to Host Mucin to Enhance Probiotic Effects

dc.contributor.authorDeng, Zhaoxien
dc.contributor.authorDai, Tianen
dc.contributor.authorZhang, Wenmingen
dc.contributor.authorZhu, Junlien
dc.contributor.authorLuo, Xin M.en
dc.contributor.authorFu, Dongyanen
dc.contributor.authorLiu, Jianxinen
dc.contributor.authorWang, Haifengen
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.date.accessioned2021-01-04T12:53:34Zen
dc.date.available2021-01-04T12:53:34Zen
dc.date.issued2020-12-21en
dc.date.updated2020-12-24T15:29:33Zen
dc.description.abstractThe ability to adhere to the intestinal mucus layer is an important property of probiotic bacteria. <i>Lactobacillus reuteri</i> strains ZJ615 and ZJ617 show low and high adhesion, respectively, to intestinal epithelial cells. In this study, we quantified bacterial cell wall-associated glyceraldehyde-3-phosphate dehydrogenases (cw-GAPDH) and bacterial cell membrane permeability in both strains using immunoblotting and flow cytometry, respectively. Highly adhesive <i>L. reuteri</i> ZJ617 possessed significantly more cw-GAPDH, higher cell membrane permeability, and significantly higher adhesive ability toward mucin compared with low-adhesive <i>L. reuteri</i> ZJ615. In vitro adhesion studies and analysis of interaction kinetics using the Octet, the system revealed significantly decreased interaction between <i>L. reuteri</i> and mucin when mucin was oxidized when bacterial surface proteins were removed when bacteria were heat-inactivated at 80 &deg;C for 30 min, and when the interaction was blocked with an anti-GAPDH antibody. SWISS-MODEL analysis suggested intensive interactions between mucin glycans (GalNAc&alpha;1-O-Ser, GalNAc&alpha;Ser, and Gal&beta;3GalNAc) and GAPDH. Furthermore, in vivo studies revealed significantly higher numbers of bacteria adhering to the jejunum, ileum, and colon of piglets orally inoculated with <i>L. reuteri</i> ZJ617 compared with those inoculated with <i>L. reuteri</i> ZJ615; this led to a significantly decreased rate of diarrhea in piglets inoculated with <i>L. reuteri</i> ZJ617. In conclusion, there are strong correlations among the abundance of cw-GAPDH in <i>L. reuteri</i>, the ability of the bacterium to adhere to the host, and the health benefits of this probiotic.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDeng, Z.; Dai, T.; Zhang, W.; Zhu, J.; Luo, X.M.; Fu, D.; Liu, J.; Wang, H. Glyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion of Lactobacillus reuteri to Host Mucin to Enhance Probiotic Effects. Int. J. Mol. Sci. 2020, 21, 9756.en
dc.identifier.doihttps://doi.org/10.3390/ijms21249756en
dc.identifier.urihttp://hdl.handle.net/10919/101728en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLactobacillus reuterien
dc.subjectGAPDHen
dc.subjectadhesionen
dc.subjectmucinen
dc.subjectintestineen
dc.titleGlyceraldehyde-3-Phosphate Dehydrogenase Increases the Adhesion of Lactobacillus reuteri to Host Mucin to Enhance Probiotic Effectsen
dc.title.serialInternational Journal of Molecular Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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