Characterization of the inositol monophosphatase gene family in Arabidopsis

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dc.contributor.authorNourbakhsh, Aidaen
dc.contributor.authorCollakova, Evaen
dc.contributor.authorGillaspy, Glenda E.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2017-01-04T22:10:35Zen
dc.date.available2017-01-04T22:10:35Zen
dc.date.issued2015-01-09en
dc.description.abstractSynthes is of myo-inositol is crucial in multicellular eukaryote for production of phosphatidylinositol and inositol phosphate signaling molecules. The myo-inositol monophosphatase (IMP) enzyme is required for the synthesis of myo-inositol, breakdown of inositol (1,4,5)-trisphosphate, a second messenger involved in Ca2+ signaling, and synthesis of L-galactose, a precursor of ascorbic acid. Two myo-inositol monophosphatase-like (IMPL) genes in Arabidopsis encode chloroplast proteins with homology to the prokaryotic IMPs and one of these, IMPL2, can complement a bacterial histidinol 1-phosphate phosphatase mutant defective in histidine synthesis, indicating an important role for IMPL2 in amino acid synthesis. To delineate how this small gene family functions in inositol synthesis and metabolism, we sought to compare recombinant enzyme activities, expression patterns, and impact of genetic loss-of-function mutations for each. Our data show that purified IMPL2 protein is an active histidinol-phosphate phosphatase enzyme in contrast to the IMPL1 enzyme, which has the ability to hydrolyze D-galactose 1-phosphate, and D-myo-inositol 1-phosphate, a breakdown product of D-inositol (1,4,5) trisphosphate. Expression studies indicated that all three genes are expressed in multiple tissues, however, IMPL1 expression is restricted to above-ground tissues only. Identification and characterization of impl1 and impl2 mutants revealed no viable mutants for IMPL1, while two different impl2 mutants were identified and shown to be severely compromised in growth, which can be rescued by histidine. Analyses of metabolite levels in impl2 and complemented mutants reveals impl2 mutant grow this impacted by alterations in the histidine biosynthesis pathway, but does not impact myo-inositol synthesis. Together, these data indicate that IMPL2 functions in the histidine biosynthetic pathway, while IMP and IMPL1 catalyze the hydrolysis of inositol- and galactose-phosphates in the plant cell.en
dc.description.versionPublished versionen
dc.format.extent? - ? (14) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fpls.2014.00725en
dc.identifier.issn1664-462Xen
dc.identifier.urihttp://hdl.handle.net/10919/73945en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000348026600001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPlant Sciencesen
dc.subjecthistidineen
dc.subjectinositolen
dc.subjecthistidinol phosphataseen
dc.subjectinositol monophosphataseen
dc.subjectIMPL2en
dc.subjectHISTIDINOL-PHOSPHATE PHOSPHATASEen
dc.subjectLILIUM-LONGIFLORUMen
dc.subjectCHLAMYDOMONAS-EUGAMETOSen
dc.subjectASCORBATE BIOSYNTHESISen
dc.subjectHIGHER PLANTSen
dc.subjectMYO-INOSITOLen
dc.subjectMETABOLISMen
dc.subjectTHALIANAen
dc.subjectMYOINOSITOLen
dc.subjectENCODESen
dc.titleCharacterization of the inositol monophosphatase gene family in Arabidopsisen
dc.title.serialFrontiers in Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Plant Pathology, Physiology, & Weed Scienceen
pubs.organisational-group/Virginia Tech/All T&R Facultyen

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