Evaluating the role of the fission yeast cyclin B Cdc13 in cell size homeostasis

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dc.contributor.authorRogers, Jessie Michaelaen
dc.contributor.committeechairHauf, Silkeen
dc.contributor.committeememberPrice, Michael Scotten
dc.contributor.committeememberWinkel, Brenda S. J.en
dc.contributor.committeememberChen, Jingen
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2021-06-16T08:00:43Zen
dc.date.available2021-06-16T08:00:43Zen
dc.date.issued2021-06-15en
dc.description.abstractMost cellular proteins retain a stable concentration as cells grow and divide, but there are exceptions. Some cell cycle regulators change in concentration with cell size. In fission yeast, Cdc13 (cyclin B), an important activator of the core cell cycle kinase Cdc2 (CDK1), increases in concentration as cells grow. It has been proposed that the concentration of such cell cycle regulators serves as a proxy for cell size and makes cell cycle progression dependent on cell size, thereby contributing to cell size homeostasis. The underlying mechanisms for the size-dependent scaling of these cell cycle regulators are poorly understood. Here, I show that Cdc13 protein concentration, but not mRNA concentration, increases with cell size. Furthermore, only the nuclear, but not the cytoplasmic, fraction of Cdc13 increases in concentration as cell size increases. Computational modeling along with half-life measurements suggests that stabilization of Cdc13 in the nucleus plays an important role in establishing this pattern. Taken together, my results suggest that Cdc13 scales with time, and therefore only indirectly—not directly—with cell size. This leaves open the possibility that Cdc13 contributes to cell size homeostasis, but in a different way than originally proposed.en
dc.description.abstractgeneralCells maintain their size very efficiently, but how they manage to do so is not well characterized. It has been suggested that cells sense their size by the size-dependent concentration changes of cell cycle proteins. I have investigated how cyclin B may serve as such a proxy for cell size in fission yeast. My data suggest that fission yeast cyclin B indirectly scales with cell size through an unknown time-based mechanism.en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.othervt_gsexam:31043en
dc.identifier.urihttp://hdl.handle.net/10919/103878en
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectCell size homeostasisen
dc.subjectsize controlen
dc.subjectCdc13en
dc.subjectcyclin Ben
dc.subjectfission yeasten
dc.titleEvaluating the role of the fission yeast cyclin B Cdc13 in cell size homeostasisen
dc.typeThesisen
thesis.degree.disciplineBiological Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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