Tube centrifugation for processing platelet-rich plasma in the horse

Fontenot, Robin L.

Tube centrifugation for processing platelet-rich plasma in the horse

dc.contributor.author	Fontenot, Robin L.	en
dc.contributor.committeechair	Dahlgren, Linda A.	en
dc.contributor.committeemember	Weinstein, Nicole M.	en
dc.contributor.committeemember	Sink, Carolyn A.	en
dc.contributor.department	Veterinary Medical Sciences	en
dc.date.accessioned	2017-04-04T19:50:40Z	en
dc.date.adate	2012-05-17	en
dc.date.available	2017-04-04T19:50:40Z	en
dc.date.issued	2012-04-18	en
dc.date.rdate	2016-09-30	en
dc.date.sdate	2012-04-27	en
dc.description.abstract	Platelet-rich plasma (PRP) is a popular treatment for equine tendon and ligament injuries; however, commercial PRP systems are expensive. Development of a safe, inexpensive alternative would make PRP therapy more widely available to horse owners. The purpose of this study was to evaluate the quality and bacteriologic safety of PRP produced by three simple, inexpensive tube centrifugation methods and compare the results to a commercial system. Citrated blood collected from 26 normal horses was processed by four methods: blood collection tubes centrifuged at 1200 and 2000 x g, a 50ml conical tube, and a commercial system. Platelet and cell counts and mean platelet volume (MPV) in whole blood and PRP were determined using an automated hematology analyzer. Results were analyzed using mixed model ANOVA with post-hoc comparisons (MPV and fold change for RBC, WBC, and platelets) and binary logistic generalized estimating equations with horse as a blocking factor (absolute numbers of WBC, and platelets). Aerobic and anaerobic cultures were performed. Significance was set at p<0.05. Mean platelet concentrations ranged from 1.55 to 2.58 fold. The conical tube method produced the highest number of PRP samples with platelet concentrations of greater than 2.5-fold and within the clinically acceptable range of >250,000 platelets/?l. WBC counts were lowest using the commercial system and unacceptably high using the red top methods. The incidence of bacterial contamination was low (2.1%). Based on these results, the conical tube method may be a suitable alternative to commercial PRP systems in cases with budgetary constraints.	en
dc.description.degree	Master of Science	en
dc.identifier.other	etd-04272012-143958	en
dc.identifier.sourceurl	http://scholar.lib.vt.edu/theses/available/etd-04272012-143958/	en
dc.identifier.uri	http://hdl.handle.net/10919/76971	en
dc.language.iso	en_US	en
dc.publisher	Virginia Tech	en
dc.rights	In Copyright	en
dc.rights.uri	http://rightsstatements.org/vocab/InC/1.0/	en
dc.subject	platelet concentrate	en
dc.subject	Platelet-rich plasma	en
dc.title	Tube centrifugation for processing platelet-rich plasma in the horse	en
dc.type	Thesis	en
dc.type.dcmitype	Text	en
thesis.degree.discipline	Veterinary Medical Sciences	en
thesis.degree.grantor	Virginia Polytechnic Institute and State University	en
thesis.degree.level	masters	en
thesis.degree.name	Master of Science	en