Z-form extracellular DNA is a structural component of the bacterial biofilm matrix

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dc.contributor.authorBuzzo, John R.en
dc.contributor.authorDevaraj, Aishwaryaen
dc.contributor.authorGloag, Erin S.en
dc.contributor.authorJurcisek, Joseph A.en
dc.contributor.authorRobledo-Avila, Franken
dc.contributor.authorKesler, Theresaen
dc.contributor.authorWilbanks, Kathrynen
dc.contributor.authorMashburn-Warren, Laurenen
dc.contributor.authorBalu, Sabarathnamen
dc.contributor.authorWickham, Josephen
dc.contributor.authorNovotny, Laura A.en
dc.contributor.authorStoodley, Paulen
dc.contributor.authorBakaletz, Lauren O.en
dc.contributor.authorGoodman, Steven D.en
dc.date.accessioned2022-12-14T20:38:28Zen
dc.date.available2022-12-14T20:38:28Zen
dc.date.issued2021-11-11en
dc.date.updated2022-12-14T19:09:28Zen
dc.description.abstractBiofilms are community architectures adopted by bacteria inclusive of a self-formed extracellular matrix that protects resident bacteria from diverse environmental stresses and, in many species, incorporates extracellular DNA (eDNA) and DNABII proteins for structural integrity throughout biofilm development. Here, we present evidence that this eDNA-based architecture relies on the rare Z-form. Z-form DNA accumulates as biofilms mature and, through stabilization by the DNABII proteins, confers structural integrity to the biofilm matrix. Indeed, substances known to drive B-DNA into Z-DNA promoted biofilm formation whereas those that drive Z-DNA into B-DNA disrupted extant biofilms. Importantly, we demonstrated that the universal bacterial DNABII family of proteins stabilizes both bacterial- and host-eDNA in the Z-form in situ. A model is proposed that incorporates the role of Z-DNA in biofilm pathogenesis, innate immune response, and immune evasion.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.cell.2021.10.010en
dc.identifier.eissn1097-4172en
dc.identifier.issn0092-8674en
dc.identifier.issue23en
dc.identifier.orcidGloag, Erin [0000-0001-8895-3444]en
dc.identifier.otherNIHMS1748889en
dc.identifier.otherPMC8595767en
dc.identifier.otherS0092-8674(21)01220-4 (PII)en
dc.identifier.urihttp://hdl.handle.net/10919/112891en
dc.identifier.volume184en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/34735796en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectDNABII proteinsen
dc.subjectDNase resistanceen
dc.subjectZ-DNAen
dc.subjectBiofilm matrixen
dc.subjectExtracellular DNAen
dc.subjectInfectious Diseasesen
dc.subject2 Aetiologyen
dc.subject2.2 Factors relating to the physical environmenten
dc.subjectInfectionen
dc.subject.meshAnimalsen
dc.subject.meshAntibody Specificityen
dc.subject.meshBacteriaen
dc.subject.meshBacterial Proteinsen
dc.subject.meshBiofilmsen
dc.subject.meshCell Lineen
dc.subject.meshChinchillaen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDNA, Cruciformen
dc.subject.meshDeoxyribonucleasesen
dc.subject.meshExtracellular Matrixen
dc.subject.meshExtracellular Spaceen
dc.subject.meshExtracellular Trapsen
dc.subject.meshHumansen
dc.subject.meshTetradecanoylphorbol Acetateen
dc.titleZ-form extracellular DNA is a structural component of the bacterial biofilm matrixen
dc.title.serialCellen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dc.type.otherResearch Support, N.I.H., Extramuralen
dc.type.otherResearch Support, Non-U.S. Gov'ten
dcterms.dateAccepted2021-10-12en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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