The behavior and effects of Brucella abortus rough strain RB51 in mice and cattle

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dc.contributor.authorBuhrman, Dianne L.en
dc.contributor.committeechairSchurig, Gerhardt G.en
dc.contributor.committeememberSriranganathan, Nammalwaren
dc.contributor.committeememberElgert, Klaus D.en
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T21:50:25Zen
dc.date.adate2012-11-21en
dc.date.available2014-03-14T21:50:25Zen
dc.date.issued1989-07-05en
dc.date.rdate2012-11-21en
dc.date.sdate2012-11-21en
dc.description.abstractBrucella abortus st. RB51 is a rough mutant of smooth st. 2308 devoid of O-side chain and resistant to rifampin. The purpose of this investigation was to study the behavior and effects of viable st. RB51 organisms in inoculated mice and cattle and to further substantiate the lack of O-side chain antigens in this strain. A single injection of live st. RB51 persisted in BALB/C mice up to 28 days. A secondary exposure was cleared in 7-21 days. One or 2 injections of st. RB51 did not induce detectable titers of anti-O-side chain antibodies, although antibody titers to st. RB51 whole cell and cytoplasmic antigens were detected. Mice infected with st. RB51 alone or followed by infection with st. 2308, demonstrated a very strong reaction to a 14-18 Kd antigen which was believed to be the core of the LPS complex. When st. RB51 was administered after injection of st. 2308 the response to the core determinants were inhibited. One vaccination with st. RB51 was able to significantly protect mice against challenge with st. 2308 at one and four weeks post challenge. Two st. RB51 vaccinations were able to protect mice as well as one vaccination at one week post challenge but protection increased by four weeks post challenge. Strain RB5l was able to survive in cattle a for at least twenty-two days. The organism remained stable, rifampin resistant, and may have induced minor amounts of transient anti-O-side chain antibodies in some cows late in the experiments.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 130 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-11212012-040055en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11212012-040055/en
dc.identifier.urihttp://hdl.handle.net/10919/45940en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1989.B837.pdfen
dc.relation.isformatofOCLC# 20432925en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1989.B837en
dc.subject.lcshBrucellosis in cattleen
dc.subject.lcshBrucellosisen
dc.titleThe behavior and effects of Brucella abortus rough strain RB51 in mice and cattleen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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