Optical Scanning Holography for 3-D Imaging of Fluorescent Objects in Turbid Media

Abstract

A holographic recording method using an optical heterodyne 2-D scanning technique for 3-D imaging of fluorescent objects in turbid media is described and experimentally demonstrated. For the first time, 3-D imaging of fluorescentobjects in turbid media by a holographic method is achieved, and the diffused photon rejecting process through a heterodyne technique is analyzed. We also propose and realize a multiplexing and a digital decoding method for removing twin-image noise in optical scanning holography. The holographic method studied can be applied to 3-D biomedical imaging of fluorescent objects in turbid media as well as diffusely reflecting objects.