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Changes in Skeletal Muscle Sarcoplasmic Reticulum Function in Adult and Aged Fisher 344 Brown x Norway Rats

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dc.contributor.authorRabon, Karma Melisaen
dc.contributor.committeechairWilliams, Jay H.en
dc.contributor.committeememberSchlenker, Eleanor D.en
dc.contributor.committeememberRedican, Kerry J.en
dc.contributor.committeememberGray, Festus Gailen
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2014-03-14T20:51:51Zen
dc.date.adate1997-06-27en
dc.date.available2014-03-14T20:51:51Zen
dc.date.issued1997-06-16en
dc.date.rdate1998-11-10en
dc.date.sdate1997-06-16en
dc.description.abstractThe decline of physical ability that occurs with aging has been linked to reduced skeletal muscle function. It has been theorized that Ca2+ uptake and release by the sarcoplasmic reticulum (SR) is altered with aging. Data pertaining to the actual structural and functional changes of SR due to aging are limited; thus, this theory has not yet been fully proven. The purpose of this research was to determine if SR function is altered as a result of aging in the following muscles: soleus, plantaris, and diaphragm. The soleus is composed of slow twitch muscle fibers. The plantaris is composed of fast twitch muscle fibers, and the diaphragm is composed of both slow and fast twitch muscle fibers. Fisher 344 Brown x Norway Cross Rats were used as subjects for this project. A total of 12 animals were used: six in group 12 months and six in group 27 months. A Jasco CAF-110 Fluorometer and fura-2 were used to determine the rate of Ca2+ uptake and release by isolated SR vesicles. In the aged animals, mass of the soleus was reduced by 22%, while the plantaris was reduced by 23%, and the diaphragm by 15%. However, these differences were eliminated when masses were normalized by body mass. In all three muscles examined, the rates of Ca2+ uptake were not significantly different between the young and aged animals. Rates of Ca2+ release, however, were reduced by 30% in both the plantaris and diaphragm of the aged animals. These results suggest that SR function is altered in "fast" muscles of the rat. It is possible that changes in SR Ca release may contribute to diminished muscle function and also lead to the decline in physical ability of older adults.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-52797-16510en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-52797-16510/en
dc.identifier.urihttp://hdl.handle.net/10919/36800en
dc.publisherVirginia Techen
dc.relation.haspartintro.pdfen
dc.relation.haspartthesis2.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectageden
dc.subjectadult, sacroplasmic reticulumen
dc.titleChanges in Skeletal Muscle Sarcoplasmic Reticulum Function in Adult and Aged Fisher 344 Brown x Norway Ratsen
dc.typeThesisen
thesis.degree.disciplineHuman Nutrition, Foods, and Exerciseen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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