The Development of an In-situ Mud Floc Microscope Imaging Device and In-situ Floc Observations from the Lowermost Mississippi River

Mud makes up a large fraction of sediment transported within rivers to the coasts. Predicting where mud will settle is complicated by the cohesive nature of silts and clays, which can combine to form larger aggregates known as flocs. The size and density, and consequently, the settling velocity, of flocs is highly dynamic and depends on factors such as turbulence levels within the flow and biogeochemical components of the water and sediment. To better predict where mud will deposit, more observations of flocs while in their natural environment is required to better understand the controls on when, where, and to what degree mud is flocculated. However, the need for more field observations is complicated by the dynamic and fragile nature of flocs. This necessitates the need for developing in-situ observation methods to ensure that measured floc sizes are representative of their in-situ size, and not a result of sampling methods. In this thesis, a new instrument for in-situ observation of flocs is presented. In addition, two methods using the data collected from the instrument allow the user to: (1) identify sand within the particle data using a machine learning algorithm, and (2) estimate the mass suspended sediment concentration of the mud and sand fractions of suspended sediment independently. Results from using the instrument in the lowermost Mississippi River reveal differences in floc sizes over the water column, and by season. In addition, a unique observation of flocs in the presence of a salt wedge is presented. Overall, the instrument provided the first known observations of flocs within the Mississippi River, and provides a start to better understanding controls on floc sizes within the fluvial environment.