Antibacterial activity and therapeutic efficacy of Fl-PRPRPL-5, a cationic amphiphilic polyproline helix, in a mouse model of staphylococcal skin infection

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dc.contributor.authorThangamani, Shankaren
dc.contributor.authorNepal, Manishen
dc.contributor.authorChmielewski, Jeanen
dc.contributor.authorSeleem, Mohamed N.en
dc.date.accessioned2020-09-21T16:10:06Zen
dc.date.available2020-09-21T16:10:06Zen
dc.date.issued2015-01-01en
dc.date.updated2020-09-21T16:10:02Zen
dc.description.abstractThe antibacterial activities and therapeutic efficacy of the cationic, unnatural proline-rich peptide Fl-PRPRPL-5 were evaluated against multidrug-resistant Staphylococcus aureus in a mouse model of skin infection. Fl-PRPRPL-5 showed potent activity against all clinical isolates of S. aureus tested, including methicillin- and vancomycin-resistant S. aureus (MRSA and VRSA, respectively). Fl-PRPRPL-5 was also superior in clearing established in vitro biofilms of S. aureus and Staphylococcus epidermidis, compared with the established antimicrobials mupirocin and vancomycin. Additionally, topical treatment of an MRSA-infected wound with Fl-PRPRPL-5 enhanced wound closure and significantly reduced bacterial load. Finally, 0.5% Fl-PRPRPL-5 significantly reduced the levels of the inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) in wounds induced by MRSA skin infection. In conclusion, the results of this study suggest the potential application of Fl-PRPRPL-5 in the treatment of staphylococcal skin infections.en
dc.description.versionPublished versionen
dc.format.extentPages 5749-5754en
dc.format.extent6 page(s)en
dc.format.mediumElectronic-eCollectionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.2147/DDDT.S94505en
dc.identifier.eissn1177-8881en
dc.identifier.issn1177-8881en
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458]en
dc.identifier.otherdddt-9-5749 (PII)en
dc.identifier.pmid26543355 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/100018en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherDove Medical Pressen
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectChemistry, Medicinalen
dc.subjectPharmacology & Pharmacyen
dc.subjectantimicrobial peptidesen
dc.subjectStaphylococcus aureusen
dc.subjectbiofilmsen
dc.subjectanti-inflammatoryen
dc.subjectskin infectionen
dc.subjectAUREUSen
dc.subjectPEPTIDESen
dc.subjectCOMPLICATIONSen
dc.subjectINFLAMMATIONen
dc.subjectBACTERIAen
dc.subjectBIOFILMen
dc.subjectALPHAen
dc.subjectRISKen
dc.subject1115 Pharmacology and Pharmaceutical Sciencesen
dc.subject.meshSkinen
dc.subject.meshAnimalsen
dc.subject.meshBiofilmsen
dc.subject.meshStaphylococcus epidermidisen
dc.subject.meshStaphylococcal Skin Infectionsen
dc.subject.meshDisease Models, Animalen
dc.subject.meshFluoresceinsen
dc.subject.meshPeptidesen
dc.subject.meshTumor Necrosis Factor-alphaen
dc.subject.meshOligopeptidesen
dc.subject.meshInflammation Mediatorsen
dc.subject.meshInterleukin-6en
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshInterleukin-1betaen
dc.subject.meshMethicillin-Resistant Staphylococcus aureusen
dc.titleAntibacterial activity and therapeutic efficacy of Fl-PRPRPL-5, a cationic amphiphilic polyproline helix, in a mouse model of staphylococcal skin infectionen
dc.title.serialDrug Design Development and Therapyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Techen

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