Bovine parvovirus and bovine enterovirus in mixed infections

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dc.contributor.authorDorsey, Ralph Benjaminen
dc.contributor.committeechairBates, Robert C.en
dc.contributor.committeememberTolin, Sue A.en
dc.contributor.committeememberClaus, George Williamen
dc.contributor.departmentMicrobiologyen
dc.date.accessioned2014-03-14T21:39:41Zen
dc.date.adate2010-07-07en
dc.date.available2014-03-14T21:39:41Zen
dc.date.issued1975-08-15en
dc.date.rdate2010-07-07en
dc.date.sdate2010-07-07en
dc.description.abstractBovine fetal spleen cells synchronized with 2mM hydroxyurea were infected with bovine parvovirus and bovine enterovirus in order to study the events occurring when DNA and RNA viruses mixedly infect single cells. The objectives of this research were threefold. First, to determine the effects of single infection of synchronized bovine fetal spleen cells by bovine parvovirus and bovine enterovirus on cellular macromolecular syntheses. Second, to study the effect of simultaneous infection of synchronized cells by bovine parvovirus and bovine enterovirus. Third, to investigate the interactions which occur when synchronized cells are pre-infected with bovine parvovirus and superinfected with bovine enterovirus. Single infection of cells with bovine parvovirus upon release from hydroxyurea does not affect cellular macromolecular syntheses until 8 hr after infection; whereas, single infection with bovine enterovirus results in a rapid decrease in the rates of total DNA, RNA, and protein synthesis by 2 hr after infection. In simultaneously infected cells, the enterovirus replication is not inhibited while the level of parvovirus is severely reduced. However, in cells pre-infected with bovine parvovirus and super-infected with bovine enterovirus, the replication of both viruses is dramatically decreased. It can be seen from the results obtained in the study of two protocols of mixed infection, that many different virus-host interactions and virus-virus interactions can occur in a mixed infection. The time sequence of infection of the two viruses determines what interactions take place.en
dc.description.degreeMaster of Scienceen
dc.format.extent106 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-07072010-020319en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07072010-020319/en
dc.identifier.urihttp://hdl.handle.net/10919/43566en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1975.D67.pdfen
dc.relation.isformatofOCLC# 20836160en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1975.D67en
dc.subject.lcshCattle -- Healthen
dc.titleBovine parvovirus and bovine enterovirus in mixed infectionsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineMicrobiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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