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Quantification of total T-cell receptor diversity by flow cytometry and spectratyping

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dc.contributor.authorCiupe, Stanca M.en
dc.contributor.authorDevlin, Blythe H.en
dc.contributor.authorMarkert, Mary L.en
dc.contributor.authorKepler, Thomas B.en
dc.contributor.departmentMathematicsen
dc.date.accessioned2017-01-22T01:57:35Zen
dc.date.available2017-01-22T01:57:35Zen
dc.date.issued2013-08-06en
dc.description.abstractBackground T-cell receptor diversity correlates with immune competency and is of particular interest in patients undergoing immune reconstitution. Spectratyping generates data about T-cell receptor CDR3 length distribution for each BV gene but is technically complex. Flow cytometry can also be used to generate data about T-cell receptor BV gene usage, but its utility has not been compared to or tested in combination with spectratyping. Results Using flow cytometry and spectratype data, we have defined a divergence metric that quantifies the deviation from normal of T-cell receptor repertoire. We have shown that the sample size is a sensitive parameter in the predicted flow divergence values, but not in the spectratype divergence values. We have derived two ways to correct for the measurement bias using mathematical and statistical approaches and have predicted a lower bound in the number of lymphocytes needed when using the divergence as a substitute for diversity. Conclusions Using both flow cytometry and spectratyping of T-cells, we have defined the divergence measure as an indirect measure of T-cell receptor diversity. We have shown the dependence of the divergence measure on the sample size before it can be used to make predictions regarding the diversity of the T-cell receptor repertoire.en
dc.description.versionPublished versionen
dc.format.extent? - ? (12) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Immunology. 2013 Aug 06;14(1):35en
dc.identifier.doihttps://doi.org/10.1186/1471-2172-14-35en
dc.identifier.issn1471-2172en
dc.identifier.urihttp://hdl.handle.net/10919/74402en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherBiomed Centralen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000322999700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderStanca M Ciupe et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectImmunologyen
dc.subjectIMMUNOLOGYen
dc.subjectREPERTOIRE DIVERSITYen
dc.subjectTHYMUS TRANSPLANTATIONen
dc.subjectDIGEORGE-SYNDROMEen
dc.subjectRECONSTITUTIONen
dc.subjectANTIGENen
dc.subjectRECOGNITIONen
dc.titleQuantification of total T-cell receptor diversity by flow cytometry and spectratypingen
dc.title.serialBMC Immunologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen
pubs.organisational-group/Virginia Tech/Science/Mathematicsen

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