The Effect of Wine Matrix Ingredients on 3-Alkyl-2-methoxypyrazines Measurements by Headspace Solid-Phase Microextraction (HS-SPME)

dc.contributor.authorHartmann, Peter J.en
dc.contributor.committeechairMcNair, Harold M.en
dc.contributor.committeecochairZoecklein, Bruce W.en
dc.contributor.committeememberTaylor, Larry T.en
dc.contributor.departmentChemistryen
dc.date.accessioned2014-03-14T20:33:06Zen
dc.date.adate2003-04-15en
dc.date.available2014-03-14T20:33:06Zen
dc.date.issued2002-12-02en
dc.date.rdate2004-04-15en
dc.date.sdate2003-04-03en
dc.description.abstractThe effect of wine matrix ingredients and conditions on the headspace (HS) sampling of 3-alkyl-2-methoxypyrazines was investigated with solid-phase microextraction (SPME) and capillary gas chromatography, using a nitrogen phosphorus detector. Changes in the recovery of 3-ethyl-, isopropyl-, sec-butyl-, and isobutyl-2-methoxypyrazines from the static headspace of synthetic wine matrices spiked with 5mg/L of each analyte were investigated and reported as a function of SPME fiber type, extraction time, and temperature. The influence of pH, ethanol, phenolics, and ground oak was studied. DVB/Carboxen?/PDMS SPME fibers at an extraction temperature of 50°C for 30 minutes with 30% (w/v) added sodium chloride resulted in the highest analyte recoveries. Although, PDMS (100 micron) SPME fibers at an extraction temperature of 35°C for 30 minutes with 30% (w/v) added sodium chloride resulted in the lower analyte recoveries, the fiber remained functional after 50 to 75 analyses after other coatings deteriorated. Changing the sample ethanol concentration from 0 to 20% (v/v) resulted in an exponential decrease in the recovered analytes. Below pH 2, there was extensive loss of the analytes in the headspace. No measurable impact on alkylmethoxypyrazine headspace concentrations was observed with exposures to selected phenolics and to ground oak.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-04032003-195302en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04032003-195302/en
dc.identifier.urihttp://hdl.handle.net/10919/31616en
dc.publisherVirginia Techen
dc.relation.haspartPJH_thesis.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectAlkyl-methoxypyraxinesen
dc.subjectGas Chromatographyen
dc.subjectHeadspace Solid-phase Microextractionen
dc.subjectWineen
dc.titleThe Effect of Wine Matrix Ingredients on 3-Alkyl-2-methoxypyrazines Measurements by Headspace Solid-Phase Microextraction (HS-SPME)en
dc.typeThesisen
thesis.degree.disciplineChemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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