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FITSelect: An Invention to Select Microbial Strains Maximizing Product Formation from a Single Culture Without High-Throughput Screening

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dc.contributor.authorZhou, Ruien
dc.contributor.committeechairSenger, Ryan S.en
dc.contributor.committeememberCollakova, Evaen
dc.contributor.committeememberZhang, Chenming Mikeen
dc.contributor.committeememberBarone, Justin R.en
dc.contributor.departmentBiological Systems Engineeringen
dc.date.accessioned2017-04-04T19:49:40Zen
dc.date.adate2011-09-14en
dc.date.available2017-04-04T19:49:40Zen
dc.date.issued2011-08-08en
dc.date.rdate2016-10-24en
dc.date.sdate2011-08-16en
dc.description.abstractIn metabolic engineering of prokaryotes, combinatorial approaches have developed recently that induce random genetic perturbations to achieve a desired cell phenotype. A screening strategy follows the randomized genetic manipulations to select strain(s) with the more optimal phenotype of interest. This screening strategy is often divided into two categories: (i) a growth competition assay and (ii) selection by high-throughput screening. The growth competition assay involves culturing strains together. The strain with the highest growth rate will ultimately dominate the culture. This strategy is ideal for selecting strain with cellular fitness (e.g., solvent tolerance), but it does not work for selecting a strain that can over-produce a product (e.g., an amino acid). For the case of selecting highly productive phenotypes, high-throughput screening is used. This method analyzes strains individually and is costly and time-consuming. In this research, a synthetic genetic circuit was developed to select highly productive phenotypes using a growth competition assay rather than high-throughput screening. This novel system is called Feed-back Inhibition of Transcription for Growth Selection (FITSelect), and it uses a natural feedback inhibition mechanism in the L-arginine production pathway to select strains (transformed with a random genomic library) that can over-produce L-arginine in E. coli DH10B. With FITSelect, the cell can thrive in the growth competition assay when L-arginine is over-produced (i.e., growth is tied to L-arginine production). Cell death or reduced growth results if L-arginine is not over-produced by the cell. This system was created by including an L-arginine concentration responsive argF promoter to control a ccdB cell death gene in the FITSelect system. The effects of ccdB were modulated by the antidote ccdA gene under control of an L-tryptophan responsive trp promoter. Several insights and construction strategies were required to build a system that ties the growth rate of the cell to L-arginine concentrations.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-08162011-203224en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08162011-203224/en
dc.identifier.urihttp://hdl.handle.net/10919/76843en
dc.language.isoen_USen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectsynthetic biologyen
dc.subjectgrowth competition assayen
dc.subjectcombinatorial methodsen
dc.subjectmetabolic engineeringen
dc.titleFITSelect: An Invention to Select Microbial Strains Maximizing Product Formation from a Single Culture Without High-Throughput Screeningen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiological Systems Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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