Validation and Characterization of Maize Multiple Disease Resistance QTL

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dc.contributor.authorMartins, Lais B.en
dc.contributor.authorRucker, Elizabethen
dc.contributor.authorThomason, Wade E.en
dc.contributor.authorWisser, Randall J.en
dc.contributor.authorHolland, James B.en
dc.contributor.authorBalint-Kurti, Peter J.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-12-20T18:33:28Zen
dc.date.available2019-12-20T18:33:28Zen
dc.date.issued2019-09en
dc.description.abstractSouthern Leaf Blight, Northern Leaf Blight, and Gray Leaf Spot, caused by ascomycete fungi, are among the most important foliar diseases of maize worldwide. Previously, disease resistance quantitative trait loci (QTL) for all three diseases were identified in a connected set of chromosome segment substitution line (CSSL) populations designed for the identification of disease resistance QTL. Some QTL for different diseases co-localized, indicating the presence of multiple disease resistance (MDR) QTL. The goal of this study was to perform an independent test of several of the MDR QTL identified to confirm their existence and derive a more precise estimate of allele additive and dominance effects. Twelve F-2:3 family populations were produced, in which selected QTL were segregating in an otherwise uniform genetic background. The populations were assessed for each of the three diseases in replicated trials and genotyped with markers previously associated with disease resistance. Pairwise phenotypic correlations across all the populations for resistance to the three diseases ranged from 0.2 to 0.3 and were all significant at the alpha level of 0.01. Of the 44 QTL tested, 16 were validated (identified at the same genomic location for the same disease or diseases) and several novel QTL/disease associations were found. Two MDR QTL were associated with resistance to all three diseases. This study identifies several potentially important MDR QTL and demonstrates the importance of independently evaluating QTL effects following their initial identification.en
dc.description.notesFunding for the work was provided by USDA-ARS, the Corn Growers' Association of North Carolina and by NSF grant #1127076 to RJW, JBH and PBK. LBM was funded by a fellowship from the NCSU Crop and Soil Science Department and Monsanto. Pioneer and Monsanto both provided space for GLS field trials for this study. We thank Cathy Herring and the staff at Central Crops Research Station for excellent field support, Shannon Sermons, Greg Marshall and Teclemariam Weldekidan for their technical support. We thank Susana Milla-Lewis and Luis Lopez-Zuniga for helpful discussion.en
dc.description.sponsorshipUSDA-ARSUnited States Department of Agriculture (USDA); Corn Growers' Association of North Carolina; NSFNational Science Foundation (NSF) [1127076]; NCSU Crop; Soil Science Department and Monsantoen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1534/g3.119.400195en
dc.identifier.eissn2160-1836en
dc.identifier.issue9en
dc.identifier.pmid31300480en
dc.identifier.urihttp://hdl.handle.net/10919/96169en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherGenetics Society of Americaen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectDiseaseen
dc.subjectMaizeen
dc.subjectQTLen
dc.subjectResistanceen
dc.subjectGenetics of Immunityen
dc.titleValidation and Characterization of Maize Multiple Disease Resistance QTLen
dc.title.serialG3-Genes Genomes Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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