In vitro propagation of Betula uber (Ashe) Fernald

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dc.contributor.authorOng, Robert C.en
dc.contributor.departmentForestryen
dc.date.accessioned2014-03-14T21:31:10Zen
dc.date.adate2009-03-12en
dc.date.available2014-03-14T21:31:10Zen
dc.date.issued1990en
dc.date.rdate2009-03-12en
dc.date.sdate2009-03-12en
dc.description.abstractDormant bud and actively growing shoot tip explants of B. uber were cultured on Murashige & Skoog (MS) and modified woody plant (WPM) basal salts supplemented with organic nutrients and benzylaminopurine (BAP). Shoot tip explants were morphogenically more responsive than dormant bud explants. The modified WPM proved to be better than MS medium for the establishment and proliferation of B. uber cultures. Shoot proliferation was greatest at 8.9 μM BAP. However, BAP at 4.4 μM produced the greatest number of shoots > 10 mm. Indolebutyric acid (IBA) was more effective than naphthaleneacetic acid (NAA) for rooting of microcuttings in vitro. Rooting was best with a low level of IBA (2.5 μM) in the culture medium. Ex vitro rooting of microcuttings using a conventional potting mix of sand-peat-perlite (1:2:1 v/v/v) was superior to all in vitro rooting treatments. Plantlets were acclimated successfully to greenhouse conditions. Genotype differences with regard to both rooting and shooting abilities were observed.en
dc.description.degreeMaster of Scienceen
dc.format.extentvi, 64 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-03122009-040812en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-03122009-040812/en
dc.identifier.urihttp://hdl.handle.net/10919/41481en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1990.O65.pdfen
dc.relation.isformatofOCLC# 23590993en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1990.O65en
dc.subject.lcshBirchen
dc.subject.lcshPlant micropropagationen
dc.titleIn vitro propagation of Betula uber (Ashe) Fernalden
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineForestryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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