Effects of available phosphorus source and concentration on performance and expression of sodium phosphate type IIb cotransporter, vitamin D-1 alpha-hydroxylase, and vitamin D-24-hydroxylase mRNA in broiler chicks

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dc.contributor.authorOmara, Islam I.en
dc.contributor.authorMou, C. T.en
dc.contributor.authorPersia, Michael E.en
dc.contributor.authorWong, Eric A.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2021-09-27T14:42:19Zen
dc.date.available2021-09-27T14:42:19Zen
dc.date.issued2020-04-01en
dc.date.updated2021-09-27T14:42:15Zen
dc.description.abstractThis experiment was conducted to examine the effect of 2 phosphorus (P) sources on broiler performance to day 14. The P bioavailability was estimated using bird performance and tibia ash measurements, whereas P digestibility, intestinal P transporter, kidney vitamin D-1α-hydroxylase, and vitamin D-24-hydroxylase mRNA abundances were also determined. Slope regression analysis was used to determine the bioavailability of dicalcium phosphate (Dical P) and nanocalcium phosphate (Nano P) with dietary available P (AvP) set to 0.20% P (control) using AvP from the major ingredients and Dical P. The experimental treatments were achieved by supplementation with either Dical P or Nano P to generate 0.24, 0.28, 0.32, and 0.36% AvP. A total of 648-day-old unsexed broiler chicks were divided into 72 birds per treatment (8 replicate cages of 9 birds). Slope regression analysis showed positive linear relationships between BW, feed intake (FI), tibia ash weight (TAW), and tibia ash percentage (TAP) with dietary Dical P and Nano P levels. Comparisons between regression slopes for Dical P and Nano P fed birds were not significantly different for BW, feed intake, tibia ash weight, and tibia ash percentage, indicating similar P bioavailability from Dical P and Nano P. There were interactions between P source and AvP for feed efficiency (FE) and apparent ileal P digestibility (AIPD). Dicalcium phosphate had greater FE than Nano P at 0.28% AvP and greater AIPD than Nano P at 0.24% AvP. The addition of AvP from Dical P and Nano P resulted in reduced sodium phosphate cotransporter mRNA abundance in the duodenum in a dose–dependent response. In the kidney, vitamin D-1α-hydroxylase mRNA abundance was greater at 0.36% Nano P compared with control, but there was no difference with Dical P. There was no difference in vitamin D-24-hydroxylase mRNA abundance between control and supplementation with Nano P or Dical P. In conclusion, Nano P and Dical P had the same bioavailability but had different effects on gene expression.en
dc.description.versionPublished versionen
dc.format.extentPages 1822-1831en
dc.format.extent10 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.psj.2019.12.035en
dc.identifier.eissn1525-3171en
dc.identifier.issn0032-5791en
dc.identifier.issue4en
dc.identifier.orcidWong, Eric [0000-0002-1891-5466]en
dc.identifier.otherS0032-5791(20)30008-0 (PII)en
dc.identifier.pmid32241462en
dc.identifier.urihttp://hdl.handle.net/10919/105081en
dc.identifier.volume99en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000525955500003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectAgriculture, Dairy & Animal Scienceen
dc.subjectAgricultureen
dc.subjectdicalcium phosphateen
dc.subjectnanophosphateen
dc.subjectphosphate transporteren
dc.subjectvitamin D hydroxylaseen
dc.subjectDIETARY NONPHYTATE PHOSPHORUSen
dc.subjectP-I DIETen
dc.subjectGROWTH-PERFORMANCEen
dc.subjectMINERAL PHOSPHORUSen
dc.subjectSMALL-INTESTINEen
dc.subjectD-RECEPTORen
dc.subjectPHYTASEen
dc.subjectDIGESTIBILITYen
dc.subjectTRANSPORTERen
dc.subjectSUPPLEMENTSen
dc.subjectDairy & Animal Scienceen
dc.subject0605 Microbiologyen
dc.subject0702 Animal Productionen
dc.subject0908 Food Sciencesen
dc.subject.meshDuodenumen
dc.subject.meshKidneyen
dc.subject.meshAnimalsen
dc.subject.meshChickensen
dc.subject.meshCalcium Phosphatesen
dc.subject.meshPhosphorus, Dietaryen
dc.subject.meshSteroid Hydroxylasesen
dc.subject.meshAvian Proteinsen
dc.subject.meshRNA, Messengeren
dc.subject.meshDieten
dc.subject.meshRandom Allocationen
dc.subject.meshBiological Availabilityen
dc.subject.meshDose-Response Relationship, Drugen
dc.subject.meshAnimal Feeden
dc.subject.meshDietary Supplementsen
dc.subject.meshSodium-Phosphate Cotransporter Proteins, Type IIben
dc.subject.meshNanoparticlesen
dc.subject.meshVitamin D3 24-Hydroxylaseen
dc.titleEffects of available phosphorus source and concentration on performance and expression of sodium phosphate type IIb cotransporter, vitamin D-1 alpha-hydroxylase, and vitamin D-24-hydroxylase mRNA in broiler chicksen
dc.title.serialPoultry Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2019-12-10en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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