Molecular targets for rapid identification of Brucellaspp

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dc.contributor.authorRatushna, Vladyslava G.en
dc.contributor.authorSturgill, David M.en
dc.contributor.authorRamamoorthy, Sheelaen
dc.contributor.authorReichow, Sherry A.en
dc.contributor.authorHe, Yongqunen
dc.contributor.authorLathigra, Rajuen
dc.contributor.authorSriranganathan, Nammalwaren
dc.contributor.authorHalling, Shirley M.en
dc.contributor.authorBoyle, Stephen M.en
dc.contributor.authorGibas, Cynthia J.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentVirginia-Maryland College of Veterinary Medicineen
dc.date.accessioned2012-08-24T12:18:37Zen
dc.date.available2012-08-24T12:18:37Zen
dc.date.issued2006-02-22en
dc.date.updated2012-08-24T12:18:37Zen
dc.description.abstractBackground Brucella is an intracellular pathogen capable of infecting animals and humans. There are six recognized species of Brucella that differ in their host preference. The genomes of the three Brucella species have been recently sequenced. Comparison of the three revealed over 98% sequence similarity at the protein level and enabled computational identification of common and differentiating genes. We validated these computational predictions and examined the expression patterns of the putative unique and differentiating genes, using genomic and reverse transcription PCR. We then screened a set of differentiating genes against classical Brucella biovars and showed the applicability of these regions in the design of diagnostic tests. Results We have identified and tested set of molecular targets that are associated in unique patterns with each of the sequenced Brucella spp. A comprehensive comparison was made among the published genome sequences of B. abortus, B. melitensis and B. suis. The comparison confirmed published differences between the three Brucella genomes, and identified subsets of features that were predicted to be of interest in a functional comparison of B. melitensis and B. suis to B. abortus. Differentiating sequence regions from B. abortus, B. melitensis and B. suis were used to develop PCR primers to test for the existence and in vitro transcription of these genes in these species. Only B. suis is found to have a significant number of unique genes, but combinations of genes and regions that exist in only two out of three genomes and are therefore useful for diagnostics were identified and confirmed. Conclusion Although not all of the differentiating genes identified were transcribed under steady state conditions, a group of genes sufficient to discriminate unambiguously between B. suis, B. melitensis, and B. abortus was identified. We present an overview of these genomic differences and the use of these features to discriminate among a number of Brucella biovars.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Microbiology. 2006 Feb 22;6(1):13en
dc.identifier.doihttps://doi.org/10.1186/1471-2180-6-13en
dc.identifier.urihttp://hdl.handle.net/10919/18954en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderVladyslava G Ratushna et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleMolecular targets for rapid identification of Brucellasppen
dc.title.serialBMC Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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