The response of 12 clones of eastern white pine (Pinus strobus L.) to ozone and nitrogen dioxide

TR Number



Journal Title

Journal ISSN

Volume Title


Virginia Tech


Grafts were made using 2-0 rootstock and scion from 12 ortets of eastern white pine (Pinus strobus L.) growing at the Radford Army Ammunition Plant (RAAP). The 12 ortets represented 4 symptom severity classes (3 ortets/class) ranging from trees with > 25% of their crowns exhibiting necrotic tipburn (Class I) to those with healthy crowns (Class IV). Grafts were made in spring 1976 and ramets were grown in a greenhouse drawing charcoal filtered air. Each treatment was performed twice, on separate days for a total of 10 ramets/clone/ treatment. Five ramets/clone were used in each 6 hour treatment. The current years growth was 7-10 weeks old when treated. The treatments were as follows: 1) 0₃-10 pphm, 2) 0₃-30 pphm, 3) NO₂-10 pphm, 4) NO₂-30 pphm, 5) 0₃-10+NO₂ -10 pphm, 6) 0₃-10+NO₂ -30 pphm, 7) no pollutant. The fumigation chamber was a modified open-top field chamber located indoors. Environmental conditions during the 14 treatments averaged: 26 C, 64% RH, and 16 Klux. The ramets were evaluated prior to fumigation and then 2, 7, and 14 days thereafter for visible symptoms. The overall injury was generally light with only 11% of the clone treatment combinations exhibiting injury on > 25% of the needle fascicles. Clones I-1 and I-2 were the most sensitive clones while clones III-1, IV-2 and to a lesser degree clones III-3 and IV-1 were tolerant. These results agree with field ratings of eastern white pine sensitivity at the RAAP and provide the first step in the development of an air pollution bioindicator system at the installation.