Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation
dc.contributor.author | Zhang, Wei | en |
dc.contributor.author | Cline, Mark A. | en |
dc.contributor.author | Liu, Dongmin | en |
dc.contributor.author | Gilbert, Elizabeth R. | en |
dc.contributor.department | Animal and Poultry Sciences | en |
dc.date.accessioned | 2017-01-06T15:49:43Z | en |
dc.date.available | 2017-01-06T15:49:43Z | en |
dc.date.issued | 2013-10-01 | en |
dc.description.abstract | ZBED6 was identified as a transcription factor that affects muscle mass and fat deposition in pigs. Mechanisms mediating effects on fat mass are unclear. The objective was to determine the effect of ZBED6 mRNA knockdown on 3T3-L1 preadipocyte differentiation and gene expression. Differentiation was associated with increased mRNA abundance of CEBP/α (P < 0.05), CEBP/β (P < 0.05), CEBP/δ (P < 0.05), FASN (P < 0.05), PPARγ (P < 0.05), and SREBP-1 (P < 0.05), and decreased abundance of PREF-1 (P < 0.05). Knockdown of ZBED6 was not associated with changes in mRNA abundance of selected genes, lipid accumulation, lipid droplet size, or cell number. These results suggest that ZBED6 does not play a major role in preadipocyte differentiation. | en |
dc.description.version | Published version | en |
dc.format.extent | 251 - 255 page(s) | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.doi | https://doi.org/10.4161/adip.26028 | en |
dc.identifier.issn | 2162-3945 | en |
dc.identifier.issue | 4 | en |
dc.identifier.uri | http://hdl.handle.net/10919/73995 | en |
dc.identifier.volume | 2 | en |
dc.language.iso | en | en |
dc.relation.uri | http://www.ncbi.nlm.nih.gov/pubmed/24052901 | en |
dc.rights | Creative Commons Attribution-NonCommercial 3.0 Unported | en |
dc.rights.holder | The Author(s) | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/ | en |
dc.subject | 3T3-L1 | en |
dc.subject | IGF2 | en |
dc.subject | RNAi | en |
dc.subject | ZBED6 | en |
dc.subject | adipogenesis | en |
dc.subject | proliferation | en |
dc.title | Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation | en |
dc.title.serial | Adipocyte | en |
dc.type | Article - Refereed | en |
dc.type.dcmitype | Text | en |
pubs.organisational-group | /Virginia Tech | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciences | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exercise | en |
pubs.organisational-group | /Virginia Tech/All T&R Faculty | en |
pubs.organisational-group | /Virginia Tech/Faculty of Health Sciences | en |
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