Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation

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dc.contributor.authorZhang, Weien
dc.contributor.authorCline, Mark A.en
dc.contributor.authorLiu, Dongminen
dc.contributor.authorGilbert, Elizabeth R.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2017-01-06T15:49:43Zen
dc.date.available2017-01-06T15:49:43Zen
dc.date.issued2013-10-01en
dc.description.abstractZBED6 was identified as a transcription factor that affects muscle mass and fat deposition in pigs. Mechanisms mediating effects on fat mass are unclear. The objective was to determine the effect of ZBED6 mRNA knockdown on 3T3-L1 preadipocyte differentiation and gene expression. Differentiation was associated with increased mRNA abundance of CEBP/α (P < 0.05), CEBP/β (P < 0.05), CEBP/δ (P < 0.05), FASN (P < 0.05), PPARγ (P < 0.05), and SREBP-1 (P < 0.05), and decreased abundance of PREF-1 (P < 0.05). Knockdown of ZBED6 was not associated with changes in mRNA abundance of selected genes, lipid accumulation, lipid droplet size, or cell number. These results suggest that ZBED6 does not play a major role in preadipocyte differentiation.en
dc.description.versionPublished versionen
dc.format.extent251 - 255 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.4161/adip.26028en
dc.identifier.issn2162-3945en
dc.identifier.issue4en
dc.identifier.urihttp://hdl.handle.net/10919/73995en
dc.identifier.volume2en
dc.language.isoenen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/24052901en
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.holderThe Author(s)en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.subject3T3-L1en
dc.subjectIGF2en
dc.subjectRNAien
dc.subjectZBED6en
dc.subjectadipogenesisen
dc.subjectproliferationen
dc.titleKnockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiationen
dc.title.serialAdipocyteen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exerciseen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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