Suspended Micro/Nanofiber Hierarchical Scaffolds for Studying Cell Mechanobiology

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dc.contributor.authorWang, Jien
dc.contributor.committeechairNain, Amrinder S.en
dc.contributor.committeememberTurner, S. Richarden
dc.contributor.committeememberRiffle, Judy S.en
dc.contributor.departmentMacromolecular Science and Engineeringen
dc.date.accessioned2017-04-04T19:49:58Zen
dc.date.adate2015-03-27en
dc.date.available2017-04-04T19:49:58Zen
dc.date.issued2015-02-11en
dc.date.rdate2016-10-18en
dc.date.sdate2015-02-20en
dc.description.abstractExtracellular matrix (ECM) is a fibrous natural cell environment, possessing complicated micro-and nano- architectures, which provides signaling cues and influences cell behavior. Mimicking this three dimensional environment in vitro is a challenge in developmental and disease biology. Here, suspended multilayer hierarchical nanofiber assemblies fabricated using the non-electrospinning STEP (Spinneret based Tunable Engineered Parameter) fiber manufacturing technique with controlled fiber diameter (microns to less than 100 nm), orientation and spacing in single and multiple layers are demonstrated as biological scaffolds. Hierarchical nanofiber assemblies were developed to control single cell shape (shape index from 0.15 to 0.57), nuclei shape (shape index 0.75 to 0.99) and focal adhesion cluster length (8-15 micrometer). To further investigate single cell-ECM biophysical interactions, nanofiber nets fused in crisscross patterns were manufactured to measure the "inside out" contractile forces of single mesenchymal stem cells (MSCs). The contractile forces (18-320 nano Newton) were found to scale with fiber structural stiffness (2 -100 nano Newton/micrometer). Cells were observed to shed debris on fibers, which were found to exert forces (15-20 nano Newton). Upon CO? deprivation, cells were observed to monotonically reduce cell spread area and contractile forces. During the apoptotic process, cells exerted both expansive and contractile forces. The platform developed in this study allows a wide parametric investigation of biophysical cues which influence cell behaviors with implications in tissue engineering, developmental biology, and disease biology.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-02202015-113826en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-02202015-113826/en
dc.identifier.urihttp://hdl.handle.net/10919/76884en
dc.language.isoen_USen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectsingle cell forcesen
dc.subjectnanofiber manufacturingen
dc.subjectcell geometryen
dc.subjecthierarchical scaffoldsen
dc.titleSuspended Micro/Nanofiber Hierarchical Scaffolds for Studying Cell Mechanobiologyen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineMacromolecular Science and Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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