Pseudomonas coronafaciens sp. nov., a new phytobacterial species diverse from Pseudomonas syringae

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dc.contributor.authorDutta, Bhabeshen
dc.contributor.authorGitaitis, Ronalden
dc.contributor.authorAgarwal, Gauraven
dc.contributor.authorCoutinho, Teresaen
dc.contributor.authorLangston, David B.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.contributor.departmentVirginia Agricultural Experiment Stationen
dc.date.accessioned2019-05-31T14:08:24Zen
dc.date.available2019-05-31T14:08:24Zen
dc.date.issued2018-12-06en
dc.description.abstractWe propose Pseudomonas coronafaciens sp. nov. as a new species in genus Pseudomonas, which is diverse from P. syringae. We also classified strains from onions which are responsible for yellow bud (YB) disease as P. coronafaciens. Sequencing of 16S rRNA gene and multi-locus sequence analysis (MLSA) of housekeeping genes (gyrB, rpoD, gltA and gap1 genes) for the P. syringae pv. coronafaciens strains along with other strains of P. syringae pathovars resulted in a distinct cluster separate from other P. syringae pathovars. Based on DNA-DNA relatedness, pathotype strain of P. syringae pv. coronafaciens (CFBP 2216(PT)) exhibited <= 35.5% similarity with the pathotype strains of P. syringae pv. syringae (CFBP 1392(PT), 4702(T)) but exhibited >= 90.6% with the YB strains (YB 12-1, YB 12-4, YB 09-1). Also, the YB strains (YB 12-1, YB 12-4, YB 09-1) were able to infect only onion but not oat, rye and Italian ryegrass (common hosts for P. syrinage pv. coronafaciens). Contrastingly, P. syringae pv. coronafaciens strains (NCPPB 600(PT), ATCC 19608, Pcf 83-300) produced typical halo blight symptoms on oat, rye and Italian rye grass but did not produce any symptoms on onion. These results provide evidence that P. syringae pv. coronafaciens should be elevated to a species level and the new YB strains may potentially be a novel pathovar of hereto proposed P. coronafaciens species.en
dc.description.notesThis work was supported in part by Vidalia Onion Committee. There was no additional external funding received for this study.en
dc.description.sponsorshipVidalia Onion Committeeen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0208271en
dc.identifier.eissn1932-6203en
dc.identifier.issue12en
dc.identifier.othere0208271en
dc.identifier.pmid30521563en
dc.identifier.urihttp://hdl.handle.net/10919/89657en
dc.identifier.volume13en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titlePseudomonas coronafaciens sp. nov., a new phytobacterial species diverse from Pseudomonas syringaeen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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