Sex-Linked Growth Disorder and Aberrant Pituitary Gene Expression in Nestin-Cre-Mediated Egr1 Conditional Knockout Mice
| dc.contributor.author | Swilley, Cody | en |
| dc.contributor.author | Lin, Yu | en |
| dc.contributor.author | Zheng, Yuze | en |
| dc.contributor.author | Xu, Xiguang | en |
| dc.contributor.author | Liu, Min | en |
| dc.contributor.author | Zimmerman, Kurt | en |
| dc.contributor.author | Xie, Hehuang | en |
| dc.date.accessioned | 2023-07-28T14:49:55Z | en |
| dc.date.available | 2023-07-28T14:49:55Z | en |
| dc.date.issued | 2023-07-06 | en |
| dc.date.updated | 2023-07-28T12:21:37Z | en |
| dc.description.abstract | Genes that regulate hormone release are essential for maintaining metabolism and energy balance. <i>Egr1</i> encodes a transcription factor that regulates hormone production and release, and a decreased in growth hormones has been reported in <i>Egr1</i> knockout mice. A reduction in growth hormones has also been observed in Nestin-Cre mice, a model frequently used to study the nervous system. Currently, it is unknown how <i>Egr1</i> loss or the Nestin-Cre driver disrupt pituitary gene expression. Here, we compared the growth curves and pituitary gene expression profiles of Nestin-Cre-mediated <i>Egr1</i> conditional knockout (<i>Egr1</i>cKO) mice with those of their controls. Reduced body weight was observed in both the Nestin-Cre and <i>Egr1</i>cKO mice, and the loss of <i>Egr1</i> had a slightly more severe impact on female mice than on male mice. RNA-seq data analyses revealed that the sex-related differences were amplified in the Nestin-Cre-mediated <i>Egr1</i> conditional knockout mice. Additionally, in the male mice, the influence of <i>Egr1</i>cKO on pituitary gene expression may be overridden by the Nestin-Cre driver. Differentially expressed genes associated with the Nestin-Cre driver were significantly enriched for genes related to growth factor activity and binding. Altogether, our results demonstrate that Nestin-Cre and the loss of <i>Egr1</i> in the neuronal cell lineage have distinct impacts on pituitary gene expression in a sex-specific manner. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Swilley, C.; Lin, Y.; Zheng, Y.; Xu, X.; Liu, M.; Zimmerman, K.; Xie, H. Sex-Linked Growth Disorder and Aberrant Pituitary Gene Expression in Nestin-Cre-Mediated Egr1 Conditional Knockout Mice. Biology 2023, 12, 966. | en |
| dc.identifier.doi | https://doi.org/10.3390/biology12070966 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/115937 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | sex difference | en |
| dc.subject | Egr1 | en |
| dc.subject | Nestin | en |
| dc.subject | RNA-seq | en |
| dc.subject | pituitary | en |
| dc.subject | growth | en |
| dc.title | Sex-Linked Growth Disorder and Aberrant Pituitary Gene Expression in Nestin-Cre-Mediated Egr1 Conditional Knockout Mice | en |
| dc.title.serial | Biology | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |