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Host GRXC6 restricts Tomato yellow leaf curl virus infection by inhibiting the nuclear export of the V2 protein

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dc.contributor.authorZhao, Wenhaoen
dc.contributor.authorZhou, Yijunen
dc.contributor.authorZhou, Xuepingen
dc.contributor.authorWang, Xiaofengen
dc.contributor.authorJi, Yinghuaen
dc.date.accessioned2021-12-15T01:53:24Zen
dc.date.available2021-12-15T01:53:24Zen
dc.date.issued2021-08-01en
dc.date.updated2021-12-15T01:53:20Zen
dc.description.abstractGeminiviruses cause serious symptoms and devastating losses in crop plants. With a circular, single-stranded DNA genome, geminiviruses multiply their genomic DNA in the nucleus, requiring the nuclear shuttling of viral proteins and viral genomic DNAs. Many host factors, acting as proviral or antiviral factors, play key roles in geminivirus infections. Here, we report the roles of a tomato glutaredoxin (GRX), SlGRXC6, in the infection of Tomato yellow leaf curl virus (TYLCV), a single-component geminivirus. The V2 protein of TYLCV specifically and preferentially interacts with SlGRXC6 among the 55-member tomato GRX family that are broadly involved in oxidative stress responses, plant development, and pathogen responses. We show that overexpressed SlGRXC6 increases the nuclear accumulation of V2 by inhibiting its nuclear export and, in turn, inhibits trafficking of the V1 protein and viral genomic DNA. Conversely, the silenced expression of SlGRXC6 leads to an enhanced susceptibility to TYLCV. SlGRXC6 is also involved in symptom development as we observed a positive correlation where overexpression of SlGRXC6 promotes while knockdown of SlGRXC6 expression inhibits plant growth. We further showed that SlGRXC6 works with SlNTRC80, a tomato NADPH-dependent thioredoxin reductase, to regulate plant growth. V2 didn't interact with SlNTRC80 but competed with SlNTR80 for binding to SlGRXC6, suggesting that the V2-disrupted SlGRXC6-SlNTRC80 interaction is partially responsible for the virus-caused symptoms. These results suggest that SlGRXC6 functions as a host restriction factor that inhibits the nuclear trafficking of viral components and point out a new way to control TYLCV infection by targeting the V2-SlGRXC6 interaction.en
dc.description.versionPublished versionen
dc.format.extent25 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN e1009844 (Article number)en
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1009844en
dc.identifier.eissn1553-7374en
dc.identifier.issn1553-7366en
dc.identifier.issue8en
dc.identifier.orcidWang, Xiaofeng [0000-0002-3850-3274]en
dc.identifier.otherPPATHOGENS-D-21-00201 (PII)en
dc.identifier.pmid34398921en
dc.identifier.urihttp://hdl.handle.net/10919/107000en
dc.identifier.volume17en
dc.language.isoenen
dc.publisherPLoSen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000685186800002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectMicrobiologyen
dc.subjectParasitologyen
dc.subjectVirologyen
dc.subjectDNA-BETA SATELLITEen
dc.subjectPLANT DEVELOPMENTen
dc.subjectTRANSIENT EXPRESSIONen
dc.subjectGLUTAREDOXINSen
dc.subjectTHIOREDOXINen
dc.subjectMOVEMENTen
dc.subjectPATHWAYSen
dc.subjectDISEASEen
dc.subjectSTRESSen
dc.subjectLOCALIZATIONen
dc.subject0605 Microbiologyen
dc.subject1107 Immunologyen
dc.subject1108 Medical Microbiologyen
dc.subjectVirologyen
dc.subject.meshCell Nucleusen
dc.subject.meshLycopersicon esculentumen
dc.subject.meshPlant Proteinsen
dc.subject.meshViral Proteinsen
dc.subject.meshVirus Replicationen
dc.subject.meshPlant Diseasesen
dc.subject.meshActive Transport, Cell Nucleusen
dc.subject.meshBegomovirusen
dc.subject.meshGlutaredoxinsen
dc.titleHost GRXC6 restricts Tomato yellow leaf curl virus infection by inhibiting the nuclear export of the V2 proteinen
dc.title.serialPLOS Pathogensen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2021-07-27en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen

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