The effect of reactive oxygen species on aged skeletal muscle

dc.contributor.authorPerkins, Talayia Nayetteen
dc.contributor.committeechairWilliams, Jay H.en
dc.contributor.committeememberThomas, Elizabeth A.en
dc.contributor.committeememberMoore, David M.en
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2014-03-14T21:52:57Zen
dc.date.adate1997-08-19en
dc.date.available2014-03-14T21:52:57Zen
dc.date.issued1997-07-30en
dc.date.rdate1998-08-19en
dc.date.sdate1997-07-30en
dc.description.abstractThe production of reactive oxygen species (ROS) may be a contributor to the progression of sarcopenia. Sarcopenia is a generic term for the loss of skeletal muscle mass, quality and strength. ROS are usually produced by radiation, but are also the byproducts of aerobic metabolism. ROS have been found to mediate various pathological conditions in a variety of tissues, to cause oxidative damage to DNA, proteins, and lipids with advancing age, and is presumably a major factor contributing to changes associated with aging. The purpose of this investigation was to determine whether the sarcoplasmic reticulum (SR) of muscle from aged animals are more susceptible to the deleterious effects of ROS. Using isolated gastrocnemius SR vesicles extracted from adult (12m) and aged (27m) male Brown Norway-Fischer 344 hybrid rats, Ca2+ uptake and release measurements were obtained. The data showed that there was a 33% difference between aged and adult gastrocnemius mass. When gastrocnemius mass was corrected for body mass, the differences was ~20% between the two groups. A 20% decrease in SR Ca2+ uptake rate was noted in aged animals. HOCl also, decreased uptake by similar extents in both groups. This result suggest that the Ca2+ pump's response to ROS are similar in both groups. AgNO3 -induced and H2O2 -induced release in aged animals was 17.94 and 7.39 nmol/mg/min and in adult animals was 30.46 and 7.18 nmol/mg/min, respectively. H2O2-induced release, when expressed as a percent of AgNO3-induced release was increased in aged animals by 54%. The results suggest that the release channel of aged muscle appears to be more sensitive to ROS. In conclusion, the data support the theory that aged animal skeletal muscle is more susceptible to the adverse effects of ROS.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-71297-151953en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-71297-151953/en
dc.identifier.urihttp://hdl.handle.net/10919/46503en
dc.publisherVirginia Techen
dc.relation.haspartPRE1.PDFen
dc.relation.haspartPRE2.PDFen
dc.relation.haspartCHAP1.PDFen
dc.relation.haspartREVLITER.PDFen
dc.relation.haspartCHAP3.PDFen
dc.relation.haspartCHAP4.PDFen
dc.relation.haspartCHAP5.PDFen
dc.relation.haspartREFER.PDFen
dc.relation.haspartAPENDIXA.PDFen
dc.relation.haspartVITA.PDFen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectcalcium ATPaseen
dc.subjectsarcoplasmic reticulumen
dc.subjectAgingen
dc.titleThe effect of reactive oxygen species on aged skeletal muscleen
dc.typeThesisen
thesis.degree.disciplineHuman Nutrition, Foods, and Exerciseen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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