Ultrarapid and ultrasensitive detection of SARS-CoV-2 antibodies in COVID-19 patients via a 3D-printed nanomaterial-based biosensing platform

dc.contributor.authorAli, Md. Azaharen
dc.contributor.authorZhang, George Feien
dc.contributor.authorHu, Chunshanen
dc.contributor.authorYuan, Binen
dc.contributor.authorJahan, Sanjidaen
dc.contributor.authorKitsios, Georgios D.en
dc.contributor.authorMorris, Alisonen
dc.contributor.authorGao, Shou-Jiangen
dc.contributor.authorPanat, Rahulen
dc.date.accessioned2023-01-20T14:43:58Zen
dc.date.available2023-01-20T14:43:58Zen
dc.date.issued2022-12-01en
dc.date.updated2023-01-19T20:44:26Zen
dc.description.abstractRapid detection of antibodies during infection and after vaccination is critical for the control of infectious outbreaks, understanding immune response, and evaluating vaccine efficacy. In this manuscript, we evaluate a simple ultrarapid test for SARS-CoV-2 antibodies in COVID-19 patients, which gives quantitative results (i.e., antibody concentration) in 10–12 s using a previously reported nanomaterial-based three-dimensional (3D)-printed biosensing platform. This platform consists of a micropillar array electrode fabricated via 3D printing of aerosolized gold nanoparticles and coated with nanoflakes of graphene and specific SARS-CoV-2 antigens, including spike S1, S1 receptor-binding domain (RBD) and nucleocapsid (N). The sensor works on the principle of electrochemical transduction, where the change of sensor impedance is realized by the interactions between the viral proteins attached to the sensor electrode surface and the antibodies. The three sensors were used to test samples from 17 COVID-19 patients and 3 patients without COVID-19. Unlike other serological tests, the 3D sensors quantitatively detected antibodies at a concentration as low as picomole within 10–12 s in human plasma samples. We found that the studied COVID-19 patients had higher concentrations of antibodies to spike proteins (RBD and S1) than to the N protein. These results demonstrate the enormous potential of the rapid antibody test platform for understanding patients' immunity, disease epidemiology and vaccine efficacy, and facilitating the control and prevention of infectious epidemics.en
dc.description.versionPublished versionen
dc.format.extentPages 5808-5826en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/jmv.28075en
dc.identifier.eissn1096-9071en
dc.identifier.issn0146-6615en
dc.identifier.issue12en
dc.identifier.pmid35981973en
dc.identifier.urihttp://hdl.handle.net/10919/113302en
dc.identifier.volume94en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/35981973en
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subject3D biosensoren
dc.subjectCOVID-19 antibodiesen
dc.subjectSARS-CoV-2en
dc.subjectadditive manufacturingen
dc.subjectgraphene oxideen
dc.subjectrapid antibody detectionen
dc.subjectLungen
dc.subjectBioengineeringen
dc.subjectImmunizationen
dc.subjectVaccine Relateden
dc.subjectBiotechnologyen
dc.subjectBiodefenseen
dc.subjectNanotechnologyen
dc.subjectEmerging Infectious Diseasesen
dc.subjectPreventionen
dc.subjectInfectious Diseasesen
dc.subjectInfectionen
dc.subject3 Good Health and Well Beingen
dc.subject.meshHumansen
dc.subject.meshGraphiteen
dc.subject.meshGolden
dc.subject.meshAntibodies, Viralen
dc.subject.meshBiosensing Techniquesen
dc.subject.meshMetal Nanoparticlesen
dc.subject.meshSpike Glycoprotein, Coronavirusen
dc.subject.meshPrinting, Three-Dimensionalen
dc.subject.meshCOVID-19en
dc.subject.meshSARS-CoV-2en
dc.titleUltrarapid and ultrasensitive detection of SARS-CoV-2 antibodies in COVID-19 patients via a 3D-printed nanomaterial-based biosensing platformen
dc.title.serialJournal of Medical Virologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2022-08-17en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Animal Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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