Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii

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dc.contributor.authorBennett, Lisa Tracyen
dc.contributor.committeechairDean, Dennis R.en
dc.contributor.committeememberChen, Jiann-Shinen
dc.contributor.committeememberLarson, Timothy J.en
dc.contributor.committeememberJohnson, John L.en
dc.contributor.committeememberSitz, Thomas O.en
dc.contributor.departmentAnaerobic Microbiologyen
dc.date.accessioned2014-03-14T21:28:37Zen
dc.date.adate2013-02-06en
dc.date.available2014-03-14T21:28:37Zen
dc.date.issued1989-12-15en
dc.date.rdate2013-02-06en
dc.date.sdate2013-02-06en
dc.description.abstractNitrogenase-catalyzed nitrogen fixation is a biochemically and genetically complex process requiring the participation of a number of different nif (nitrogen fixation) gene products. The nifF (electron transport), nifA (nif gene regulation) and nifE (FeMo-cofactor biosynthesis) genes from <i>Azotobacter vinelandii</i> were genetically analyzed. The nucleotide sequence of the nifF gene, which encodes a flavodoxin, was determined. Specific mutation strains indicated that in <i>A vinelandii</i> flavodoxin is not the unique physiological electron donor to nitrogenase. The nifF gene appears to be constitutively expressed but under nitrogen fixing conditions nifF gene expression is stimulated.en
dc.description.degreeMaster of Scienceen
dc.format.extentix, 142 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-02062013-040043en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-02062013-040043/en
dc.identifier.urihttp://hdl.handle.net/10919/40938en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1989.B466.pdfen
dc.relation.isformatofOCLC# 21766921en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1989.B466en
dc.subject.lcshKlebsiella pneumoniaeen
dc.subject.lcshMutagenesisen
dc.titleGenetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandiien
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineAnaerobic Microbiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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