Detailed characterization of the UMAMIT proteins provides insight into their evolution, amino acid transport properties, and role in the plant

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dc.contributor.authorZhao, Chengsongen
dc.contributor.authorPratelli, Rejaneen
dc.contributor.authorYu, Shien
dc.contributor.authorShelley, Bretten
dc.contributor.authorCollakova, Evaen
dc.contributor.authorPilot, Guillaumeen
dc.date.accessioned2022-01-18T13:55:18Zen
dc.date.available2022-01-18T13:55:18Zen
dc.date.issued2021-09-30en
dc.date.updated2022-01-18T13:55:15Zen
dc.description.abstractAmino acid transporters play a critical role in distributing amino acids within the cell compartments and between plant organs. Despite this importance, relatively few amino acid transporter genes have been characterized and their role elucidated with certainty. Two main families of proteins encode amino acid transporters in plants: the amino acid-polyamine-organocation superfamily, containing mostly importers, and the UMAMIT (usually multiple acids move in and out transporter) family, apparently encoding exporters, totaling 63 and 44 genes in Arabidopsis, respectively. Knowledge of UMAMITs is scarce, based on six Arabidopsis genes and a handful of genes from other species. To gain insight into the role of the members of this family and provide data to be used for future characterization, we studied the evolution of the UMAMITs in plants, and determined the functional properties, the structure, and localization of the 47 Arabidopsis UMAMITs. Our analysis showed that the AtUMAMITs are essentially localized at the tonoplast or the plasma membrane, and that most of them are able to export amino acids from the cytosol, confirming a role in intra- and intercellular amino acid transport. As an example, this set of data was used to hypothesize the role of a few AtUMAMITs in the plant and the cell.en
dc.description.versionAccepted versionen
dc.format.extentPages 6400-6417en
dc.format.extent18 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/jxb/erab288en
dc.identifier.eissn1460-2431en
dc.identifier.issn0022-0957en
dc.identifier.issue18en
dc.identifier.orcidCollakova, Eva [0000-0003-3476-6701]en
dc.identifier.orcidPilot, Guillaume [0000-0001-7520-1059]en
dc.identifier.other6314604 (PII)en
dc.identifier.pmid34223868en
dc.identifier.urihttp://hdl.handle.net/10919/107746en
dc.identifier.volume72en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000709557800026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectPlant Sciencesen
dc.subjectAmino acid transporteren
dc.subjectArabidopsis thalianaen
dc.subjectfacilitatoren
dc.subjectheterologous expressionen
dc.subjectmembrane transporten
dc.subjectphylogenyen
dc.subjectSaccharomyces cerevisiaeen
dc.subjectsubcellular localizationen
dc.subjectuniporteren
dc.subjectMETABOLITE CONCENTRATIONSen
dc.subjectENDOPLASMIC-RETICULUMen
dc.subjectSUBCELLULAR VOLUMESen
dc.subjectWEB SERVERen
dc.subjectARABIDOPSISen
dc.subjectEXPRESSIONen
dc.subjectGENEen
dc.subjectPREDICTIONen
dc.subjectTONOPLASTen
dc.subjectTOPOLOGYen
dc.subject0604 Geneticsen
dc.subject0607 Plant Biologyen
dc.subject0703 Crop and Pasture Productionen
dc.subjectPlant Biology & Botanyen
dc.subject.meshVacuolesen
dc.subject.meshArabidopsisen
dc.subject.meshAmino Acidsen
dc.subject.meshAmino Acid Transport Systemsen
dc.subject.meshPlant Proteinsen
dc.subject.meshArabidopsis Proteinsen
dc.subject.meshPhylogenyen
dc.titleDetailed characterization of the UMAMIT proteins provides insight into their evolution, amino acid transport properties, and role in the planten
dc.title.serialJournal of Experimental Botanyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2021-06-11en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen

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