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Capture filtration for concentration and detection of selected microorganisms in milk

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dc.contributor.authorByrne, Robert Duaneen
dc.contributor.committeechairBishop, J. Russellen
dc.contributor.committeememberDuncan, Susan E.en
dc.contributor.committeememberEigel, William N. IIIen
dc.contributor.committeememberHackney, Cameron Rajen
dc.contributor.committeememberJones, Gerald M.en
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2014-03-14T21:14:23Zen
dc.date.adate2008-06-06en
dc.date.available2014-03-14T21:14:23Zen
dc.date.issued1994-05-04en
dc.date.rdate2008-06-06en
dc.date.sdate2008-06-06en
dc.description.abstractThe effectiveness of an adsorption filter in retaining bacteria present in milk was examined. Skim milk and whole milk (100ml) were separately filtered through a 47mm adsorption filter. No significant change in total solids, total fat, and solids-not-fat percentages of skim and whole milk permeates was observed after filtration. Adsorption of Pseudomonas fluorescens at target concentrations of 103 , 102 , and 101 cells/ml was determined in 100ml of dairy standard methods buffer, nutrient broth, whole milk, and skim milk. The average percentage bacterial retentions were 95 ± 5.5%, 95 ± 2.6%, 28 ± 22.1%, and 62 ± 15.5%, respectively. A treatment was developed for milk to increase the bacterial retention of ~ fluorescens after filtration. The preferred treatment for 100ml of skim milk involved the following final concentrations (v/v): 0.80% disodium ethylenediamine-tetraacetic acid, 0.02% sodium dodecyl sulfate, pH to 7.5 with 1N sodium hydroxide. The average bacterial retention of ~ fluorescens using the treatment was 91 ± 7.1%. Enumeration of bacteria adsorbed to the filter was then conducted using impedance microbiology. When milk was inoculated with ~ fluorescens at target concentrations of 103 , 102 , and 101 cells/ml, an average log bacterial increase of 1.4 ± 0.1 (25x) was obtained. This method will allow for rapid detection of microorganisms in milk by increasing microbial load in the tested sample and eliminating the need for pre-enrichment.en
dc.description.degreePh. D.en
dc.format.extentx, 80 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06062008-170931en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06062008-170931/en
dc.identifier.urihttp://hdl.handle.net/10919/38443en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1994.B976.pdfen
dc.relation.isformatofOCLC# 30924539en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1994.B976en
dc.subject.lcshMilk -- Microbiologyen
dc.titleCapture filtration for concentration and detection of selected microorganisms in milken
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineFood Science and Technologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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