Recovery of Xylitol from Fermentation of Model Hemicellulose Hydrolysates Using Membrane Technology

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dc.contributor.authorAffleck, Richard Peteren
dc.contributor.committeechairAgblevor, Foster Aryien
dc.contributor.committeememberCundiff, John S.en
dc.contributor.committeememberChen, Jiann-Shinen
dc.contributor.committeememberGlasser, Wolfgang G.en
dc.contributor.departmentBiological Systems Engineeringen
dc.date.accessioned2014-03-14T20:30:20Zen
dc.date.adate2001-01-12en
dc.date.available2014-03-14T20:30:20Zen
dc.date.issued2000-12-12en
dc.date.rdate2002-01-12en
dc.date.sdate2001-01-10en
dc.description.abstractXylitol can be produced from xylose or hemicellulose hydrolysates by either chemical reduction or microbial fermentation. Current technology for commercial production is based on chemical reduction of xylose or hemicellulose, and xylitol is separated and purified by chromatographic methods. The resultant product is very expensive because of the extensive purification procedures. Microbial production of xylitol is being researched as an alternative method for xylitol production. Apart from the chromatographic separation method and activated carbon treatment, no other separation method has been proposed for the separation of xylitol from the fermentation broth. Membrane separation was proposed as an alternative method for the recovery of xylitol from the fermentation broth because it has the potential for energy savings and higher purity. A membrane separation unit was designed, constructed, tested, and successfully used to separate xylitol from the fermentation broth. Eleven membranes were investigated for xylitol separation from the fermentation broth. A 10,000 nominal molecular weight cutoff (MWCO) polysulfone membrane was found to be the most effective for the separation and recovery of xylitol. The membrane allowed 82.2 to 90.3% of xylitol in the fermentation broth to pass through while retaining 49.2 to 53.6% of the Lowry's method positive material (such as oligopeptides and peptides). Permeate from the 10,000 MWCO membrane was collected and crystallized. Crystals were analyzed by HPLC for xylitol and impurities and determined to have purity up to 90.3%.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-01102001-121200en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-01102001-121200/en
dc.identifier.urihttp://hdl.handle.net/10919/30873en
dc.publisherVirginia Techen
dc.relation.haspartturninetdII.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectxylitolen
dc.subjectreverse osmosisen
dc.subjectultrafiltrationen
dc.subjectnanofiltrationen
dc.subjectfermentationen
dc.titleRecovery of Xylitol from Fermentation of Model Hemicellulose Hydrolysates Using Membrane Technologyen
dc.typeThesisen
thesis.degree.disciplineBiological Systems Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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