Microphysiometry Studies of Rapid Binding of Insulin-Like Growth Factor I by Parental and Transfected Mammary Epithelial Cell Lines

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dc.contributor.authorRobinson, Rose Marieen
dc.contributor.committeechairForsten-Williams, Kimberlyen
dc.contributor.committeememberDavis, Richey M.en
dc.contributor.committeememberAkers, Robert Michaelen
dc.contributor.departmentChemical Engineeringen
dc.date.accessioned2014-03-14T20:46:37Zen
dc.date.adate1998-11-13en
dc.date.available2014-03-14T20:46:37Zen
dc.date.issued1998-10-16en
dc.date.rdate1999-11-13en
dc.date.sdate1998-10-12en
dc.description.abstractBreast cancer is a leading cause of cancer death of women in the U.S. today. Members of the family of insulin-like growth factors (IGFs) are proposed to play a major role in the development and subsequent uncontrolled proliferation of breast cancer cells. Insulin-like growth factor-I (IGF-I) is known to be a potent mitogen for mammary epithelial cells. IGF-I acts by binding to cell surface receptors, thereby stimulating a cascade of events leading to cell division. In the interest of interrupting the effect of IGF-I on cancerous mammary epithelial cells, an understanding of how IGF-I behaves in the presence of other extracellular components is needed. This study examines the IGF-I response of SV40-IGF-I, an immortalized bovine mammary epithelial cell line which secretes IGF-I constitutively. The microphysiometer allows real-time sampling of cellular activity by measuring the excretion of protons from a sample of cells stimulated by IGF-I binding. The contributions of other factors in enhancing or suppressing stimulation can be compared by examining the pH response of cells exposed to IGF-I in the presence of these factors. We present data showing the stimulatory effect of IGF-I in a dose dependent manner on the SV40-IGF-I cell line. In addition, we compare IGF-I stimulation with stimulation by long R3IGF-I, a substituted analogue of IGF-I having a reduced binding affinity for the IGF binding proteins. We examine the effect of insulin-like binding protein-3 (IGFBP-3) both in the presence and absence of IGF-I, finding no IGF-I independent effect in the rapid binding experiment and no effect on stimulation of IGFBP-3 pre-incubated cells by subsequent IGF-I challenge. This is of particular interest due to recent work demonstrating an IGF-independent IGFBP-3 response in a number of cell lines. Binding studies to correlate with the rapid binding stimulation show binding of the IGFBP-3 molecule with high affinity to a small number of surface receptors on the SV40-IGF-I cell. Analysis of the extracellular environment and the components contributing to the binding of IGF-I to the cell membrane receptor will provide information for the development of interventions to slow or interrupt the process of IGF-I binding and therefore cancer growth. Optimization of the Cytosensor(r) Microphysiometer System for the (transfected) SV40-IGF-I and the (parental) MAC-T cell lines was achieved to continue comparison studies of autocrine and paracrine stimulation of bovine mammary epithelial cells by IGF-I. This work was supported by the Whitaker Foundation Biomedical Engineering Grant.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-101198-121758en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-101198-121758/en
dc.identifier.urihttp://hdl.handle.net/10919/35363en
dc.publisherVirginia Techen
dc.relation.haspartChapter1.pdfen
dc.relation.haspartChapter3.pdfen
dc.relation.haspartVita.pdfen
dc.relation.haspartChapter4.pdfen
dc.relation.haspartAppendix_A.pdfen
dc.relation.haspartBibliography.pdfen
dc.relation.haspartChapter6.pdfen
dc.relation.haspartChapter5.pdfen
dc.relation.haspartCONTENT_DOC.pdfen
dc.relation.haspartACKn_DOC.pdfen
dc.relation.haspartAbstract.pdfen
dc.relation.haspartthesistitle.pdfen
dc.relation.haspartChapter2.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectInsulin-Like Growth Factor II (IGF-II)en
dc.subjectInsulin-Like Growth Factor I (IGF-I)en
dc.subjectSV40-IGF-Ien
dc.subjectMAC-Ten
dc.titleMicrophysiometry Studies of Rapid Binding of Insulin-Like Growth Factor I by Parental and Transfected Mammary Epithelial Cell Linesen
dc.typeThesisen
thesis.degree.disciplineChemical Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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