Biomarkers of Lipid Oxidation in the Oral Cavity

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dc.contributor.authorVereb, Heather A.en
dc.contributor.committeechairDietrich, Andrea M.en
dc.contributor.committeememberAgah, Masouden
dc.contributor.committeememberVikesland, Peter J.en
dc.contributor.departmentEnvironmental Sciences and Engineeringen
dc.date.accessioned2017-04-04T19:49:59Zen
dc.date.adate2012-06-11en
dc.date.available2017-04-04T19:49:59Zen
dc.date.issued2011-10-14en
dc.date.rdate2016-10-18en
dc.date.sdate2011-11-13en
dc.description.abstractMeasuring lipid oxidation is useful as a means of monitoring oxidative stress, such as that induced by clinical conditions or environmental exposure. Characteristic volatile compounds, often with low threshold odors, are secondary products of lipid oxidation reactions. Metallic flavor in food and beverages has been linked with oxidation of lipids in the oral cavity. Breath, an emerging medium for analysis of internal condition, is one means of measuring the metal-induced lipid oxidation responsible for this flavor. This project analyzes the breath of human subjects, as well as lipid oxidation of in vitro samples to identify compounds responsible for producing metallic flavor, which result from the oxidation of lipids in the oral cavity. Because these analytes are found at extremely low (picomolar to nanomolar) concentrations, preconcentration of samples prior to gas chromatography-mass spectrometry analysis is crucial. This study utilizes both solid phase microextraction (SPME) and micromachined silicon micropreconcentrators to concentrate compounds in breath to optimize analysis.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-11132011-231522en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11132011-231522/en
dc.identifier.urihttp://hdl.handle.net/10919/76887en
dc.language.isoen_USen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectlipid oxidationen
dc.subjectbreath analysisen
dc.subjectmicropreconcentratoren
dc.subjectsolid phase microextraction (SPME)en
dc.subjectgas chromatographyen
dc.titleBiomarkers of Lipid Oxidation in the Oral Cavityen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineEnvironmental Sciences and Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen
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