Adsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayers

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dc.contributor.authorQian, Chenen
dc.contributor.committeechairRoman, Marenen
dc.contributor.committeecochairEsker, Alan R.en
dc.contributor.committeememberMarand, Hervé L.en
dc.contributor.departmentChemistryen
dc.date.accessioned2014-03-14T21:35:38Zen
dc.date.adate2012-06-13en
dc.date.available2014-03-14T21:35:38Zen
dc.date.issued2012-04-19en
dc.date.rdate2012-06-13en
dc.date.sdate2012-05-04en
dc.description.abstractAdsorption of xyloglucan (XG) onto thin desulfated nanocrystalline cellulose (DNC) films was studied by surface plasmon resonance spectroscopy (SPR), quartz crystal microbalance with dissipation monitoring (QCM-D), and atomic force microscopy (AFM) measurements. These studies were compared to adsorption studies of XG onto thin sulfated nanocrystalline cellulose (SNC) films and regenerated cellulose (RC) films performed by others. Collectively, these studies show the accessible surface area is the key factor for the differences in surface concentrations observed for XG adsorbed onto the three cellulose surfaces. XG penetrated into the porous nanocrystalline cellulose films. In contrast, XG was confined to the surfaces of the smooth, non-porous RC films. Surprisingly surface charge and cellulose morphology played a limited role on XG adsorption. The effect of the non-ionic surfactant Tween 80 on the adsorption of cellulase onto alkane thiol self-assembled monolayers (SAMs) on gold was also studied. Methyl (-CH3), hydroxyl (-OH) and carboxyl (-COOH) terminated SAMs were prepared. Adsorption of cellulase onto untreated and Tween 80-treated SAMs were monitored by SPR, QCM-D and AFM. The results indicated cellulase adsorption onto SAM-CH3 and SAM-COOH were driven by strong hydrophobic and electrostatic interactions, however, hydrogen bonding between cellulase and SAM-OH was weak. Tween 80 effectively hindered the adsorption of cellulase onto hydrophobic SAM-CH3 substrates. In contrast, it had almost no effect on the adsorption of cellulase onto SAM-OH and SAM-COOH substrates because of its reversible adsorption on these substrates.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05042012-155054en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05042012-155054/en
dc.identifier.urihttp://hdl.handle.net/10919/42496en
dc.publisherVirginia Techen
dc.relation.haspartQian_C_T_2012.pdfen
dc.relation.haspartQian_C_T_2012_Copyright.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectcellulaseen
dc.subjectself-assembled monolayersen
dc.subjectxyloglucanen
dc.subjectcellulose thin filmsen
dc.subjectadsorptionen
dc.titleAdsorption of Xyloglucan onto Cellulose and Cellulase onto Self-assembled Monolayersen
dc.typeThesisen
thesis.degree.disciplineChemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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