Detection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reaction

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dc.contributor.authorSchuchert, Jennifer Annen
dc.contributor.committeechairInzana, Thomas J.en
dc.contributor.committeememberBoyle, Stephen M.en
dc.contributor.committeememberSriranganathan, Nammalwaren
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T20:41:51Zen
dc.date.adate2002-08-30en
dc.date.available2014-03-14T20:41:51Zen
dc.date.issued2002-06-26en
dc.date.rdate2003-08-30en
dc.date.sdate2002-07-24en
dc.description.abstractTraditional immunological assays used to serotype Actinobacillus pleuropneumoniae have been problematic due to cross- reactivity between serotypes, particularly serotypes 6 and 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and identify serotypes 1, 2, and 8. Primers specific to the conserved capsular polysaccharide export region of A. pleuropneumoniae serotype 5 amplified a 880 bp fragment in all serotypes excluding serotype 4 or a 489 bp DNA fragment in all serotypes including serotype 4. Primers specific to the capsular polysaccharide biosynthesis regions of A. pleuropneumoniae serotypes 1, 2, and 8 amplified a 1.6 kb, a 1.7 kb, and 970 bp fragment in the respective serotype. This PCR assay detects A. pleuropneumoniae and identifies serotypes 1, 2, and 8.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-07242002-214305en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07242002-214305/en
dc.identifier.urihttp://hdl.handle.net/10919/34135en
dc.publisherVirginia Techen
dc.relation.haspartfinalThesis082602.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectMultiplex PCRen
dc.subjectserotypingen
dc.subjectActinobacillus pleuropneumoniaeen
dc.titleDetection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reactionen
dc.typeThesisen
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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