Detection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reaction
dc.contributor.author | Schuchert, Jennifer Ann | en |
dc.contributor.committeechair | Inzana, Thomas J. | en |
dc.contributor.committeemember | Boyle, Stephen M. | en |
dc.contributor.committeemember | Sriranganathan, Nammalwar | en |
dc.contributor.department | Veterinary Medical Sciences | en |
dc.date.accessioned | 2014-03-14T20:41:51Z | en |
dc.date.adate | 2002-08-30 | en |
dc.date.available | 2014-03-14T20:41:51Z | en |
dc.date.issued | 2002-06-26 | en |
dc.date.rdate | 2003-08-30 | en |
dc.date.sdate | 2002-07-24 | en |
dc.description.abstract | Traditional immunological assays used to serotype Actinobacillus pleuropneumoniae have been problematic due to cross- reactivity between serotypes, particularly serotypes 6 and 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and identify serotypes 1, 2, and 8. Primers specific to the conserved capsular polysaccharide export region of A. pleuropneumoniae serotype 5 amplified a 880 bp fragment in all serotypes excluding serotype 4 or a 489 bp DNA fragment in all serotypes including serotype 4. Primers specific to the capsular polysaccharide biosynthesis regions of A. pleuropneumoniae serotypes 1, 2, and 8 amplified a 1.6 kb, a 1.7 kb, and 970 bp fragment in the respective serotype. This PCR assay detects A. pleuropneumoniae and identifies serotypes 1, 2, and 8. | en |
dc.description.degree | Master of Science | en |
dc.identifier.other | etd-07242002-214305 | en |
dc.identifier.sourceurl | http://scholar.lib.vt.edu/theses/available/etd-07242002-214305/ | en |
dc.identifier.uri | http://hdl.handle.net/10919/34135 | en |
dc.publisher | Virginia Tech | en |
dc.relation.haspart | finalThesis082602.pdf | en |
dc.rights | In Copyright | en |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
dc.subject | Multiplex PCR | en |
dc.subject | serotyping | en |
dc.subject | Actinobacillus pleuropneumoniae | en |
dc.title | Detection of Actinobacillus Pleuropneumoniae and Identification of Serotypes 1, 2, and 8 by Multiplex Polymerase Chain Reaction | en |
dc.type | Thesis | en |
thesis.degree.discipline | Veterinary Medical Sciences | en |
thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
thesis.degree.level | masters | en |
thesis.degree.name | Master of Science | en |
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