Integration of small RNAs and transcriptome sequencing uncovers a complex regulatory network during vernalization and heading stages of orchardgrass (Dactylis glomerata L.)

Simple item page
dc.contributor.authorFeng, Guangyanen
dc.contributor.authorXu, Leien
dc.contributor.authorWang, Jianpingen
dc.contributor.authorNie, Gangen
dc.contributor.authorBushman, Bradley Shaunen
dc.contributor.authorXie, Wengangen
dc.contributor.authorYan, Haidongen
dc.contributor.authorYang, Zhongfuen
dc.contributor.authorGuan, Haoen
dc.contributor.authorHuang, Linkaien
dc.contributor.authorZhang, Xinquanen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2018-10-08T12:40:09Zen
dc.date.available2018-10-08T12:40:09Zen
dc.date.issued2018-10-03en
dc.date.updated2018-10-07T03:20:20Zen
dc.description.abstractBackground Flowering is a critical reproductive process in higher plants. Timing of optimal flowering depends upon the coordination among seasonal environmental cues. For cool season grasses, such as Dactylis glomerata, vernalization induced by low temperature provides competence to initiate flowering after prolonged cold. We combined analyses of the transcriptome and microRNAs (miRNAs) to generate a comprehensive resource for regulatory miRNAs and their target circuits during vernalization and heading stages. Results A total of 3,846 differentially expressed genes (DEGs) and 69 differentially expressed miRNAs were identified across five flowering stages. The expression of miR395, miR530, miR167, miR396, miR528, novel_42, novel_72, novel_107, and novel_123 demonstrated significant variations during vernalization. These miRNA targeted genes were involved in phytohormones, transmembrane transport, and plant morphogenesis in response to vernalization. The expression patterns of DEGs related to plant hormones, stress responses, energy metabolism, and signal transduction changed significantly in the transition from vegetative to reproductive phases. Conclusions Five hub genes, c136110_g1 (BRI1), c131375_g1 (BZR1), c133350_g1 (VRN1), c139830_g1 (VIN3), and c125792_g2 (FT), might play central roles in vernalization response. Our comprehensive analyses have provided a useful platform for investigating consecutive transcriptional and post-transcriptional regulation of critical phases in D. glomerata and provided insights into the genetic engineering of flowering-control in cereal crops.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Genomics. 2018 Oct 03;19(1):727en
dc.identifier.doihttps://doi.org/10.1186/s12864-018-5104-0en
dc.identifier.urihttp://hdl.handle.net/10919/85263en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderThe Author(s)en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIntegration of small RNAs and transcriptome sequencing uncovers a complex regulatory network during vernalization and heading stages of orchardgrass (Dactylis glomerata L.)en
dc.title.serialBMC Genomicsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
12864_2018_Article_5104.pdf
Size:
2.16 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Journal Articles, BioMed Central and SpringerOpen
Scholarly Works, School of Plant and Environmental Sciences