Pathogenic and antigenic characterization of Neospora hughesi

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dc.contributor.authorWalsh, Catherine Patriciaen
dc.contributor.committeechairLindsay, David S.en
dc.contributor.committeememberBuechner-Maxwell, Virginia A.en
dc.contributor.committeememberSriranganathan, Nammalwaren
dc.contributor.committeememberZajac, Anne M.en
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T20:37:18Zen
dc.date.adate2000-05-19en
dc.date.available2014-03-14T20:37:18Zen
dc.date.issued2000-05-02en
dc.date.rdate2001-05-19en
dc.date.sdate2000-05-17en
dc.description.abstract<I>Neospora hughesi</I> is a recently described cause of equine protozoal myeloencephalitis (EPM). In the present study, we examined the susceptibility of BALB/c gamma-interferon gene knockout (gamma-INFKO), BALB/c, CD-1, and C57BL/6 strains of mice and gerbils to infection with tachyzoites of the Nh-A1 strain of <I>N. hughesi</I>. Only the gamma-IFNKO mice developed severe clinical disease following infection with <I>N. hughesi</I>. The most severe lesions were in the hearts of these mice. Two dogs fed the brains of mice, shown to contain <I>N. hughesi</I> tissue stages by cell culture and g-IFNKO mouse bioassay, did not shed <I>N. hughesi</I> oocysts over a 23 day observation period. We report important differences between the nucleotide and deduced amino acid sequences of the dense granule proteins GRA6 and GRA7 of <I>N. hughesi and N. caninum</I>. The newly defined proteins of <I>N. hughesi</I> are referred to as NhGRA6 and NhGRA7. From analysis of the sequences we found that there is a 14.8% difference in deduced amino acid sequence between NhGRA7 and NcGRA7, and a 4% difference between NhGRA6 and NcGRA6 in areas that could be compared. This thesis supports the identification of <I>N. hughesi</I> as a separate species from <I>N. caninum</I> and describes novel methods of distinguishing between the two.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05172000-10210051en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05172000-10210051/en
dc.identifier.urihttp://hdl.handle.net/10919/32893en
dc.publisherVirginia Techen
dc.relation.hasparteverything.pdfen
dc.relation.haspartta.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectEPMen
dc.subjectNeospora hughesien
dc.subjectNeospora caninumen
dc.subjectDense granulesen
dc.subjectRodent modelsen
dc.titlePathogenic and antigenic characterization of <I>Neospora hughesi</I>en
dc.typeThesisen
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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