Novel approaches to evaluate osteoarthritis in the rabbit lateral meniscectomy model

Simple item page
dc.contributor.authorPease, Anthony P.en
dc.contributor.committeechairVeit, Hugo P.en
dc.contributor.committeememberMisra, Hara P.en
dc.contributor.committeememberJohnston, Spencer A.en
dc.contributor.committeememberRobertson, John L.en
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2011-08-06T16:01:20Zen
dc.date.adate2000-07-12en
dc.date.available2011-08-06T16:01:20Zen
dc.date.issued1997-11-20en
dc.date.rdate2001-07-12en
dc.date.sdate2000-06-16en
dc.description.abstractA rabbit lateral meniscectomy model was used to induce osteoarthritis. Separate studies were conducted to evaluate the progression of osteoarthritis and to identify possible biological markers. First, 21 male, New Zealand White rabbits were divided into 3 groups (n = 7 / group). A randomly selected left or right stifle underwent a lateral meniscectomy. The 3 groups were: corticosteroid administration, forced exercise and surgical control. An open field maze was used to assess mobility weekly. The rabbits were euthanitized 47 days after surgery. Histopathologic examination found that the lateral meniscectomy induced more severe lesions than in the non-surgical contralateral stifle. It also showed a significant sparing effect on erosion of cartilage in the corticosteroid group. The corticosteroid group, but not the exercise group, caused a significant increase in mobility (p = 0.008) compared to the surgical control. Secondly, synovial fluid was harvested from the 12 rabbits on days 0, 6, 26, 40, and 57 with surgery occurring on day 12. Trypan blue was used in the lavage fluid to estimate the volume of harvested synovial fluid. There was a significant increase in the volume harvested on day 26 (p < 0.001). Superoxide dismutase concentration in synovial fluid increased after surgery, although not significantly. These studies verify that the lateral meniscectomy model produce histopathologic lesions consistent with osteoarthritis. Furthermore, use of trypan blue appears to be a reliable concentration marker in a lavage sample to measure harvested synovial fluid.en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.otheretd-06162000-01190030en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06162000-01190030en
dc.identifier.urihttp://hdl.handle.net/10919/9869en
dc.publisherVirginia Techen
dc.relation.haspartPeaseThesis.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectsuperoxide dismutaseen
dc.subjectExerciseen
dc.subjectcorticosteroidsen
dc.subjectosteoarthritisen
dc.subjecttrypan blueen
dc.subjectopen field mazeen
dc.titleNovel approaches to evaluate osteoarthritis in the rabbit lateral meniscectomy modelen
dc.typeThesisen
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PeaseThesis.pdf
Size:
1.65 MB
Format:
Adobe Portable Document Format
Download

Collections

Masters Theses