Evaluation of a mitochondrial test for the determination of chemical toxicity

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dc.contributor.authorShannon, Robert Daviden
dc.contributor.committeechairBoardman, Gregory D.en
dc.contributor.committeememberHoehn, Robert C.en
dc.contributor.committeememberBevan, David R.en
dc.contributor.committeememberDietrich, Andrea M.en
dc.contributor.departmentEnvironmental Sciences and Engineeringen
dc.date.accessioned2014-03-14T21:37:41Zen
dc.date.adate2012-06-10en
dc.date.available2014-03-14T21:37:41Zen
dc.date.issued1988-07-15en
dc.date.rdate2012-06-10en
dc.date.sdate2012-06-10en
dc.description.abstractThe feasibility of using rat liver mitochondria respiratory parameters as a short-term toxicity test was investigated. Mitochondrial fractions were exposed to six concentrations of five chlorophenols. Respiratory parameters were measured and compared to control experiments. The toxicity of the chlorophenos, measured by the 50% uncoupling concentration (UC50), increased with increasing chloro substitution. The UC50 values for the five chlorophenols were compared to six physicochemical parameters for the same chlorophenols and high degrees of correlation were found (r >/- 0.890). The highest correlation coefficient obtained was with the octanol-water partition coefficient. UC50 values were also compared to nine currently existing short-term toxicity tests. High degrees of correlation were obtained with several of these tests, including bacterial and fish bioassays. From the results of these experiments, the measurement and use of mitochondria respiratory parameters as a short-term toxicity test appears to offer an alternative to currently used short-term toxicity tests, particularly with chemicals having physicochemical characteristics similar to mitochondria uncouplers.en
dc.description.degreeMaster of Scienceen
dc.format.extentxi, 132 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06102012-040255en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06102012-040255/en
dc.identifier.urihttp://hdl.handle.net/10919/43054en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1988.S423.pdfen
dc.relation.isformatofOCLC# 18616327en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1988.S423en
dc.subject.lcshMitochondriaen
dc.subject.lcshToxicity testingen
dc.titleEvaluation of a mitochondrial test for the determination of chemical toxicityen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineEnvironmental Sciences and Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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