An evaluation of a modified membrane filter technique for the recovery of fecal coliforms exposed to selected heavy metals

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dc.contributor.authorGayle, Benjamin P.en
dc.contributor.committeechairHoehn, Robert C.en
dc.contributor.committeememberBoardman, Gregory D.en
dc.contributor.committeememberKing, Paul H.en
dc.contributor.departmentEnvironmental Sciences and Engineeringen
dc.date.accessioned2014-03-14T21:41:23Zen
dc.date.adate2010-07-28en
dc.date.available2014-03-14T21:41:23Zen
dc.date.issued1977-12-15en
dc.date.rdate2010-07-28en
dc.date.sdate2010-07-28en
dc.description.abstractA bench study was conducted to compare two membrane filter techniques for their efficiency in recovering fecal coliforms exposed to selected heavy metals. The effects on recovery by increasing time and metal concentration were also examined. The recovery methods employed included the standard membrane filter technique (S-MF) and a modified membrane filter technique (M-MF)~ which consisted of a lactose agar overlay and a five-hour preincubation at 35 C. The heavy metals Cd, Cr, Pb, and Zn were examined, each at two concentrations, to evaluate their effect on the recovery of the I. coli test organism, after exposure for 6, 24, and 48 hours. A statistical analysis of the data found the recoveries obtained by the M-MF to be significantly greater (.0001 level) than those of the S-MF, in all cases. Time was also found to significantly effect recovery, with recoveries decreasing as time increased. A significant difference was also found between the effects of the heavy metals tested and the concentrations of metals was likewise found to significantly effect recovery, with decreased recoveries being obtained at the high concentration of each metal.en
dc.description.degreeMaster of Scienceen
dc.format.extent91 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-07282010-020123en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07282010-020123/en
dc.identifier.urihttp://hdl.handle.net/10919/43935en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1977.G395.pdfen
dc.relation.isformatofOCLC# 09249644en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1977.G395en
dc.subject.lcshFeces -- Microbiologyen
dc.subject.lcshMembrane filtersen
dc.subject.lcshWater -- Pollution -- Measurementen
dc.titleAn evaluation of a modified membrane filter technique for the recovery of fecal coliforms exposed to selected heavy metalsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineEnvironmental Sciences and Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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