Leptospira Seroprevalence Among Ugandan Slaughter Cattle: Comparison of Sero-Status With Renal Leptospira Infection

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dc.contributor.authorAlinaitwe, Lordricken
dc.contributor.authorKankya, Cloviceen
dc.contributor.authorNamanya, Dianahen
dc.contributor.authorPithua, Patricken
dc.contributor.authorDreyfus, Anouen
dc.contributor.departmentPopulation Health Sciencesen
dc.date.accessioned2021-09-29T16:35:37Zen
dc.date.available2021-09-29T16:35:37Zen
dc.date.issued2020-02-28en
dc.date.updated2021-09-29T16:35:34Zen
dc.description.abstractDespite evidence of both human and animal Leptospira exposures in Uganda, the epidemiology of the disease is still not well-investigated. Contact with animals and their environments have been pointed out as potential source of infection with Leptospira species in humans; and cattle may be an important reservoir in Uganda. In this cross-sectional study, we estimated the prevalence of anti-Leptospira antibodies by the standard microscopic agglutination test (MAT); and associated risk factors among slaughtered cattle. We also compared the performance of the MAT used in this study against a lipL32 based real time PCR (qPCR) assay previously conducted on the kidneys and urine of the same slaughter cattle as tested in this reported study. Of 500 cattle sampled, 27.8% (95% CI 23.9–32.0) tested positive (titer ≥ 100) to at least one Leptospira serovar, with the majority of seropositive cattle reacting to serovars Tarassovi (sg Tarassovi) (11.6%), Sejroe (Sg Sejroe) (7.8%), and Australis (Sg Australis) (5.2%). Older animals had 2.8 times (95% CI 1.0–8.2, p-value 0.055) greater odds of being seropositive than younger ones (<1.5 years). The sensitivity and specificity of the MAT over the qPCR were 65.9% (95% CI 50.1–79.5) and 75.9% (95% CI 71.7–79.7), respectively; with a negative predictive value of 95.8% and positive predictive value of 20.9%. In conclusion, slaughter cattle in this study were significantly exposed to pathogenic Leptospira species of mainly the Tarassovi, Sejroe, and Australis serogroups, with seroprevalence being higher among older cattle. The high specificity and negative predictive value of MAT as used in this study when compared to the qPCR assay may imply a rather strong association between seronegativity and absence of renal Leptospira infection. However, MAT predictability for renal Leptospira infection may be interpreted cautiously since predictive values of diagnostic tests are dependent on prevalence.en
dc.description.versionPublished versionen
dc.format.extent7 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 106 (Article number)en
dc.identifier.doihttps://doi.org/10.3389/fvets.2020.00106en
dc.identifier.eissn2297-1769en
dc.identifier.issn2297-1769en
dc.identifier.orcidPithua, Patrick [0000-0002-2817-5772]en
dc.identifier.pmid32185188en
dc.identifier.urihttp://hdl.handle.net/10919/105108en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000525145200001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectVeterinary Sciencesen
dc.subjectleptospirosisen
dc.subjectmicroscopic agglutination testen
dc.subjectrenal Leptospira infectionen
dc.subjectslaughter cattleen
dc.subjectseroprevalenceen
dc.subjectBOVINE LEPTOSPIROSISen
dc.subjectRISK-FACTORSen
dc.subjectPREVALENCEen
dc.subjectDIAGNOSISen
dc.subjectPIGSen
dc.subjectleptospirosisen
dc.subject0707 Veterinary Sciencesen
dc.titleLeptospira Seroprevalence Among Ugandan Slaughter Cattle: Comparison of Sero-Status With Renal Leptospira Infectionen
dc.title.serialFrontiers in Veterinary Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2020-02-11en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Population Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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