The Role of the Salmonella spvB IncF Plasmid and Its Resident Entry Exclusion Gene traS on Plasmid Exclusion

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dc.contributor.authorOluwadare, Mopelolaen
dc.contributor.authorLee, Margie D.en
dc.contributor.authorGrim, Christopher J.en
dc.contributor.authorLipp, Erin K.en
dc.contributor.authorCheng, Yingen
dc.contributor.authorMaurer, John J.en
dc.contributor.departmentBiomedical Sciences and Pathobiologyen
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2020-08-17T19:11:32Zen
dc.date.available2020-08-17T19:11:32Zen
dc.date.issued2020-05-15en
dc.description.abstractSalmonella enterica cause significant illnesses worldwide. There has been a marked increase in resistance to fluoroquinolones and beta-lactams/cephalosporins, antibiotics commonly used to treat salmonellosis. However, S. enterica serovars vary in their resistance to these and other antibiotics. The systemic virulence of some Salmonella serovars is due to a low copy number, IncF plasmid (65-100 kb) that contains the ADP-ribosylating toxin, SpvB. This virulence plasmid is present in only nine Salmonella serovars. It is possible that the spvB-virulence plasmid excludes other plasmids and may explain why antibiotic resistance is slow to develop in certain Salmonella serovars such as S. Enteritidis. The distribution of plasmid entry exclusion genes traS/traT and traY/excA are variable in Salmonella IncF and IncI plasmids, respectively and may account for differences in emergent antimicrobial resistance for some Salmonella serovars. The goal of this study is to determine the contribution of the Salmonella spvB-virulence plasmid in F-plasmid exclusion. From conjugation experiments, S. Typhimurium exhibited lower conjugation frequency with incFI and incFII plasmids when the spvB-virulence plasmid is present. Furthermore, introduction of cloned incFI traS into a "plasmidless" S. Typhimurium LT2 strain and Escherichia coli DH5 alpha excluded incFI plasmid. However, deletion of the virulence plasmid traS did not affect plasmid exclusion significantly compared to a spvB control deletion. In addition, differences in F plasmid conjugation in natural Salmonella isolates did not correlate with IncF or SpvB-virulence plasmid genotype. There appear to be other plasmid or chromosomal genes at play in plasmid exclusion that may be responsible for the slow development of antibiotic resistance in certain serovars.en
dc.description.notesThis work was supported by grants from the United States Department of Agriculture (2017-05586; VA-160130).en
dc.description.sponsorshipUnited States Department of AgricultureUnited States Department of Agriculture (USDA) [2017-05586, VA-160130]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fmicb.2020.00949en
dc.identifier.issn1664-302Xen
dc.identifier.other949en
dc.identifier.pmid32499773en
dc.identifier.urihttp://hdl.handle.net/10919/99725en
dc.identifier.volume11en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectSalmonellaen
dc.subjectvirulence plasmiden
dc.subjectantibiotic resistanceen
dc.subjectentry exclusionen
dc.subjectconjugationen
dc.titleThe Role of the Salmonella spvB IncF Plasmid and Its Resident Entry Exclusion Gene traS on Plasmid Exclusionen
dc.title.serialFrontiers In Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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Destination Area: Global Systems Science (GSS)
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