Derivation and Comparison of Androgenic and Gynogenic Monoploid Potato Families

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dc.contributor.authorCutright, Rebecca J.en
dc.contributor.committeechairVeilleux, Richard E.en
dc.contributor.committeememberBeers, Eric P.en
dc.contributor.committeememberGriffey, Carl A.en
dc.contributor.departmentHorticultureen
dc.date.accessioned2014-03-14T20:52:30Zen
dc.date.adate1998-09-09en
dc.date.available2014-03-14T20:52:30Zen
dc.date.issued1998-07-16en
dc.date.rdate2012-11-20en
dc.date.sdate1998-07-16en
dc.description.abstractMonopoloid potato (2n = 1x = 12) can be derived either paternally through anther/microspore culture or maternally through crossing with a haploid-inducing pollinator. Evidence from other genera suggests that androgenic and gynogenic haploid populations derived from the same parent may differ due to gametic selection and/or epigenetic factors. Our objectives were to derive androgenic and gynogenic monoploid populations from each of two diploid (2n = 2x = 24) Solanum phureja clones and compare their phenotypic characteristics in a greenhouse study. A haploid-inducing pollinator, S. phureja IVP101, was crossed to two selections of S. phureja (PP5 and BARD1-3). A total of 185 fruit was obtained from PP5 and 398 from BARD1-3, resulting from 85% and 65% fruit set, respectively. Seed lacking the dominant embryo spot marker carried by IVP101 were selected and germinated in vitro. From 29,300 PP5 x IVP101 seeds, 278 were spotless, resulting in 27 monoploids. Approximately 37 monoploids were obtained from the 99,500 BARD1-3 x IVP101 seeds of which 500 were spotless. In anther culture, PP5 and BARD1-3 yielded 0.16 and 1.67 embryos per anther respectively of which 51% and 44% of the regenerants were monoploid. A total of 32 anther-derived monoploids has been obtained from PP5 and 130 from BARD1-3. Rooted cuttings of 21 androgenic and 21 gynogenic PP5 monoploids were established in a greenhouse in each of three randomized complete blocks. Although the anther-derived monoploids appeared more vigorous, none of the differences between the two populations were significant. Flow cytometry revealed that anther derived progeny of PP5 and BARD1-3 had 3-8% greater nuclear DNA content than gynogenic monoploids derived from the same parental clones.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-8598-184718en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-8598-184718/en
dc.identifier.urihttp://hdl.handle.net/10919/37017en
dc.publisherVirginia Techen
dc.relation.haspartch1.pdfen
dc.relation.haspartch1_figs.pdfen
dc.relation.haspartch2.pdfen
dc.relation.haspartch2_figs.pdfen
dc.relation.haspartch2_tbls.pdfen
dc.relation.haspartch3.pdfen
dc.relation.haspartch3_figs.pdfen
dc.relation.haspartch3_tbls.pdfen
dc.relation.haspartintro.pdfen
dc.relation.haspartvita.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectPotatoen
dc.subjectAnther Cultureen
dc.subjectHaploid-inducing Pollinatoren
dc.titleDerivation and Comparison of Androgenic and Gynogenic Monoploid Potato Familiesen
dc.typeThesisen
thesis.degree.disciplineHorticultureen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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