Isolation of a novel insect-specific flavivirus with immunomodulatory effects in vertebrate systems

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dc.contributor.authorAuguste, A. Jonathanen
dc.contributor.authorLangsjoen, Rose M.en
dc.contributor.authorPorier, Danielle L.en
dc.contributor.authorErasmus, Jesse H.en
dc.contributor.authorBergren, Nicholas A.en
dc.contributor.authorBolling, Bethany G.en
dc.contributor.authorLuo, Huanleen
dc.contributor.authorSingh, Ankitaen
dc.contributor.authorGuzman, Hildaen
dc.contributor.authorPopov, Vsevolod L.en
dc.contributor.authorda Rosa, Amelia P. A. Travassosen
dc.contributor.authorWang, Tianen
dc.contributor.authorKang, Linen
dc.contributor.authorAllen, Irving C.en
dc.contributor.authorCarrington, Christine V. F.en
dc.contributor.authorTesh, Robert B.en
dc.contributor.authorWeaver, Scott C.en
dc.date.accessioned2021-12-06T18:16:13Zen
dc.date.available2021-12-06T18:16:13Zen
dc.date.issued2021-10en
dc.description.abstractWe describe the isolation and characterization of a novel insect-specific flavivirus (ISFV), tentatively named Aripo virus (ARPV), that was isolated from Psorophora albipes mosquitoes collected in Trinidad. The ARPV genome was determined and phylogenetic analyses showed that it is a dual host associated ISFV, and clusters with the main mosquito-borne flaviviruses. ARPV antigen was significantly cross-reactive with Japanese encephalitis virus serogroup antisera, with significant cross-reactivity to Ilheus and West Nile virus (WNV). Results suggest that ARPV replication is limited to mosquitoes, as it did not replicate in the sandfly, culicoides or vertebrate cell lines tested. We also demonstrated that ARPV is endocytosed into vertebrate cells and is highly immunomodulatory, producing a robust innate immune response despite its inability to replicate in vertebrate systems. We show that prior infection or coinfection with ARPV limits WNV-induced disease in mouse models, likely the result of a robust ARPV-induced type I interferon response.en
dc.description.notesThis work was supported by grants from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under Award Numbers K22AI125474 and R01AI153433 to AJA, and R01AI121452 and R24AI120942 to SCW. We thank Steven Widen, Thomas Wood and Jill Thompson for assistance with sequencing.en
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases of the National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of Allergy & Infectious Diseases (NIAID) [K22AI125474, R01AI153433, R01AI121452, R24AI120942]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.virol.2021.07.004en
dc.identifier.eissn1089-862Xen
dc.identifier.issn0042-6822en
dc.identifier.pmid34256244en
dc.identifier.urihttp://hdl.handle.net/10919/106850en
dc.identifier.volume562en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectInsect-specific flavivirusen
dc.subjectAripo virusen
dc.subjectWest nile virusen
dc.subjectFlavivirus infectionen
dc.subjectFlavivirus pathogenesisen
dc.subjectSuper-infection exclusionen
dc.titleIsolation of a novel insect-specific flavivirus with immunomodulatory effects in vertebrate systemsen
dc.title.serialVirologyen
dc.typeArticle - Refereeden
dc.type.dcmitypetexten

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Scholarly Works, Entomology
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Scholarly Works, Center for Emerging, Zoonotic, and Arthropod-borne Pathogens (CeZAP)