Design, Synthesis and Characterization of Heme-proteins: Developing Potential Catalysts for Bio-remediation

dc.contributor.authorShah, Kinjalkumar K.en
dc.contributor.committeechairZhang, Chenming Mikeen
dc.contributor.committeecochairGillespie, Joelen
dc.contributor.committeememberCundiff, John S.en
dc.contributor.committeememberBevan, David R.en
dc.contributor.departmentBiological Systems Engineeringen
dc.description.abstractThe next generation of toxic chemicals and hazardous wastes from sophisticated chemical industries will demand the environmental agencies to employ biological methods over the conventional physical and chemical remediation methods. Over the past decade, natural metallo-enzymes have been identified to degrade some of the major chemical contaminants through electron transfer pathways. However, these natural enzymes are less stable in organic solvents and they are not effective for the degradation of toxic compounds such as polychlorinated biphenyls or dioxins. This thesis explores the use of protein design approaches to produce chemically and molecularly modified enzymes, which are highly stable, possess little substrate specificity, and have higher activity than the natural enzymes. The experiments presented in this thesis make use of solid phase synthesis and site-directed mutagenesis for the synthesis and production of these enzymes and popular chromatographic techniques for their purification. The partial characterization of these proteins revealed the essential structural features of these proteins, and their catalytic activity was demonstrated by the use of peroxidase assays.en
dc.description.degreeMaster of Scienceen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.subjectPeroxidase activityen
dc.subjectCytochrome b562en
dc.subjectProtein designen
dc.subjectSolid phase peptide synthesisen
dc.subjectFour-helix bundle proteinen
dc.titleDesign, Synthesis and Characterization of Heme-proteins: Developing Potential Catalysts for Bio-remediationen
dc.typeThesisen Systems Engineeringen Polytechnic Institute and State Universityen of Scienceen


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